In vivo cryotechniques for preparation of animal tissues for immunoelectron microscopy

Shinichi Ohno, Nobuhiko Ohno, Nobuo Terada, Sei Saitoh, Yurika Saitoh, Yasuhisa Fujii

研究成果: Chapter

1 被引用数 (Scopus)

抄録

The final goal of immunohistochemical studies is that all findings examined in animal experiments should reflect the physiologically functional background. Therefore, the preservation of original components in cells and tissues is necessary for describing the functional morphology of living animal organs. It is generally accepted that morphological findings of various organs are easily modified during the conventional preparation steps. The quick-freezing method, by which resected tissues are quickly frozen, reduces morphological artifacts resulting in significant findings of native cells and tissues. However, tissues have to first be resected from living animal organs for quick-freezing. We have developed an "in vivo cryotechnique" for immunohistochemistry of some components in living animal organs. All physiological processes are immediately immobilized in the ice crystals by the "in vivo cryotechnique," and every components of the cells and tissues are maintained in situ at the time of freezing. Thus, ischemic or anoxic effects are minimized on immunohistochemical localization of the components. Another new "cryobiopsy" technique will be useful for capturing time-dependent morphological changes in the same animal including humans and for maintaining intracellular components.

本文言語English
ホスト出版物のタイトルImmunoelectron Microscopy
ホスト出版物のサブタイトルMethods and Protocols
編集者Steven Schwartzbach, Tetsuaki Osafune
ページ167-179
ページ数13
DOI
出版ステータスPublished - 01-12-2010
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
657
ISSN(印刷版)1064-3745

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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