In Vivo Identification of Protein Kinase Substrates by Kinase-Oriented Substrate Screening (KIOSS)

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Protein phosphorylation plays a critical role in the regulation of cellular function. Information on protein phosphorylation and the responsible kinases is important for understanding intracellular signaling. A method for in vivo screening of kinase substrates named KIOSS (kinase-oriented substrate screening) has been developed. This protocol provides a method that utilizes phosphoprotein-binding modules such as 14-3-3 protein, the pin1-WW domain, and the chek2-FHA domain as biological filters to successfully enrich phosphorylated proteins related to intracellular signaling rather than housekeeping and/or structural proteins. More than 1000 substrate candidates for PKA, PKC, MAPK, and Rho-kinase in HeLa cells, as well as phosphorylation downstream of D1R, NMDAR, adenosine A2a receptor, PKA, PKC, MAPK, and Rho-kinase in mouse brain slice cultures have been identified by this method. An online database named KANPHOS (Kinase-Associated Neural Phospho-Signaling) provides the phosphorylation signals identified by these studies, as well as those previously reported in the literature.

本文言語English
ページ(範囲)e60
ジャーナルCurrent protocols in chemical biology
11
1
DOI
出版ステータスPublished - 01-03-2019
外部発表はい

All Science Journal Classification (ASJC) codes

  • 医学(全般)

フィンガープリント

「In Vivo Identification of Protein Kinase Substrates by Kinase-Oriented Substrate Screening (KIOSS)」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル