TY - JOUR
T1 - Increase in dicentric chromosome formation after a single CT scan in adults
AU - Abe, Yu
AU - Miura, Tomisato
AU - Yoshida, Mitsuaki A.
AU - Ujiie, Risa
AU - Kurosu, Yumiko
AU - Kato, Nagisa
AU - Katafuchi, Atsushi
AU - Tsuyama, Naohiro
AU - Ohba, Takashi
AU - Inamasu, Tomoko
AU - Shishido, Fumio
AU - Noji, Hideyoshi
AU - Ogawa, Kazuei
AU - Yokouchi, Hiroshi
AU - Kanazawa, Kenya
AU - Ishida, Takashi
AU - Muto, Satoshi
AU - Ohsugi, Jun
AU - Suzuki, Hiroyuki
AU - Ishikawa, Tetsuo
AU - Kamiya, Kenji
AU - Sakai, Akira
PY - 2015/9/9
Y1 - 2015/9/9
N2 - Excess risk of leukemia and brain tumors after CT scans in children has been reported. We performed dicentric chromosome assay (DCAs) before and after CT scan to assess effects of low-dose ionizing radiation on chromosomes. Peripheral blood (PB) lymphocytes were collected from 10 patients before and after a CT scan. DCA was performed by analyzing either 1,000 or 2,000 metaphases using both Giemsa staining and centromere-fluorescence in situ hybridization (Centromere-FISH). The increment of DIC formation was compared with effective radiation dose calculated using the computational dosimetry system, WAZA-ARI and dose length product (DLP) in a CT scan. Dicentric chromosome (DIC) formation increased significantly after a single CT scan, and increased DIC formation was found in all patients. A good correlation between the increment of DIC formation determined by analysis of 2,000 metaphases using Giemsa staining and those by 2,000 metaphases using Centromere-FISH was observed. However, no correlation was observed between the increment of DIC formation and the effective radiation dose. Therefore, these results suggest that chromosome cleavage may be induced by one CT scan, and we recommend 2,000 or more metaphases be analyzed in Giemsa staining or Centromere-FISH for DCAs in cases of low-dose radiation exposure.
AB - Excess risk of leukemia and brain tumors after CT scans in children has been reported. We performed dicentric chromosome assay (DCAs) before and after CT scan to assess effects of low-dose ionizing radiation on chromosomes. Peripheral blood (PB) lymphocytes were collected from 10 patients before and after a CT scan. DCA was performed by analyzing either 1,000 or 2,000 metaphases using both Giemsa staining and centromere-fluorescence in situ hybridization (Centromere-FISH). The increment of DIC formation was compared with effective radiation dose calculated using the computational dosimetry system, WAZA-ARI and dose length product (DLP) in a CT scan. Dicentric chromosome (DIC) formation increased significantly after a single CT scan, and increased DIC formation was found in all patients. A good correlation between the increment of DIC formation determined by analysis of 2,000 metaphases using Giemsa staining and those by 2,000 metaphases using Centromere-FISH was observed. However, no correlation was observed between the increment of DIC formation and the effective radiation dose. Therefore, these results suggest that chromosome cleavage may be induced by one CT scan, and we recommend 2,000 or more metaphases be analyzed in Giemsa staining or Centromere-FISH for DCAs in cases of low-dose radiation exposure.
UR - https://www.scopus.com/pages/publications/84941034572
UR - https://www.scopus.com/pages/publications/84941034572#tab=citedBy
U2 - 10.1038/srep13882
DO - 10.1038/srep13882
M3 - Article
C2 - 26349546
AN - SCOPUS:84941034572
SN - 2045-2322
VL - 5
JO - Scientific reports
JF - Scientific reports
M1 - 13882
ER -