Increase in pulmonary vascular permeability caused by increased expression of Mac-1 on the surface of polymorphonuclear leukocytes

T. Tanita, C. Song, S. Ueda, Y. Hoshikawa, S. Maeda, M. Noda, T. Tabata, S. Suzuki, S. Ono, S. Fujimura

研究成果: Article

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We studied the expression of adhesion molecules on the surface of human polymorphonuclear leukocytes (PMNs). The effects of mechanical stimulation were measured with a flow cytometer and pulmonary vascular injury due to accumulation of PMNs in the lungs was assessed by a gravimetric method. The accumulation of PMNs in the lungs was studied by measuring the amount of myeloperoxidase. PMNs were stimulated by gentle agitation in a glass container for 10s, Mac-1 (CD11b/CD18) was unregulated on the surface of PMNs that were mechanically stimulated. When unstimulated PMNs were exposed to isolated rat lungs, the filtration coefficient did not change from that under baseline conditions. However, when mechanically stimulated PMNs were exposed to isolated rat lungs, the filtration coefficient was about 5 times higher than that measured at baseline. When mechanically stimulated PMNs treated with anti-CD18 antibody were used, the increase in the filtration coefficient was completely blocked. The assay of myeloperoxidase revealed that PMNs stuck to isolated rat lungs only after stimulated PMNs were added. We conclude that when the adhesiveness of PMNs is increased by mechanical stimulation, these cells adhere to pulmonary vessels and increase pulmonary vascular permeability.

元の言語English
ページ(範囲)396-401
ページ数6
ジャーナルJapanese Journal of Thoracic Diseases
35
発行部数4
出版物ステータスPublished - 12-08-1997
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

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  • これを引用

    Tanita, T., Song, C., Ueda, S., Hoshikawa, Y., Maeda, S., Noda, M., Tabata, T., Suzuki, S., Ono, S., & Fujimura, S. (1997). Increase in pulmonary vascular permeability caused by increased expression of Mac-1 on the surface of polymorphonuclear leukocytes. Japanese Journal of Thoracic Diseases, 35(4), 396-401.