Increased apoptosis of peripheral blood T cells following allogeneic hematopoietic cell transplantation

Ming Tseh Lin, Li Hui Tseng, Haydar Frangoul, Ted Gooley, Ji Pei, Alexandre Barsoukov, Yoshiki Akatsuka, John A. Hansen

研究成果: Article

60 引用 (Scopus)

抄録

Lymphopenia and immune deficiency are significant problems following allogeneic hematopoietic cell transplantation (HCT). It is largely assumed that delayed immune reconstruction is due to a profound decrease in thymus- dependent lymphopoiesis, especially in older patients, but apoptosis is also known to play a significant role in lymphocyte homeostasis. Peripheral T cells from patients who received HCT were studied for evidence of increased cell death. Spontaneous apoptosis was measured in CD3+ T cells following a 24-hour incubation using 7-amino-actinomycin D in conjunction with the dual staining of cell surface antigens. Apoptosis was significantly greater among CD3+ T cells taken from patients 19-23 days after transplantation (30.4% ± 12.5%, P < .05), and 1 year after transplantation (9.7% ± 2.8%, P < .05) compared with healthy controls (4.0% ± 1.5%). Increased apoptosis occurred preferentially in HLA (human leukocyte antigen)-DR positive cells and in both CD3+/CD4+ and CD3+/CD8+ T-cell subsets, while CD56+/CD3- natural killer cells were relatively resistant to apoptosis. The extent of CD4+ T-cell apoptosis was greater in patients with grade II-IV acute graft-versus-host disease (GVHD) (33.9% ± 11.3%) compared with grade 0-1 GVHD (14.6 ± 6.5%, P < .05). T-cell apoptosis was also greater in patients who received transplantations from HLA-mismatched donors (39.5% ± 10.4%, P < .05) or HLA- matched unrelated donors (32.1% ± 11.4%, P < .05) compared with patients who received transplantations from HLA-identical siblings (19.6% ± 6.7%). The intensity of apoptosis among CD4+ T cells was significantly correlated with a lower CD4+ T-cell count. Together, these observations suggest that activation of T cells in vivo, presumably by alloantigens, predisposes the cells to spontaneous apoptosis, and this phenomenon is associated with lymphopenla. Activation-induced T-cell apoptosis may contribute to delayed immune reconstitution following HCT. (C) 2000 by The American Society of Hematology.

元の言語English
ページ(範囲)3832-3839
ページ数8
ジャーナルBlood
95
発行部数12
出版物ステータスPublished - 15-06-2000

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T-cells
Cell Transplantation
Blood Cells
Blood
Apoptosis
T-Lymphocytes
HLA Antigens
Transplantation
Graft vs Host Disease
Grafts
Chemical activation
Lymphopoiesis
Thymus
Unrelated Donors
Lymphopenia
Isoantigens
Lymphocytes
T-Lymphocyte Subsets
Cell death
Surface Antigens

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

これを引用

Lin, M. T., Tseng, L. H., Frangoul, H., Gooley, T., Pei, J., Barsoukov, A., ... Hansen, J. A. (2000). Increased apoptosis of peripheral blood T cells following allogeneic hematopoietic cell transplantation. Blood, 95(12), 3832-3839.
Lin, Ming Tseh ; Tseng, Li Hui ; Frangoul, Haydar ; Gooley, Ted ; Pei, Ji ; Barsoukov, Alexandre ; Akatsuka, Yoshiki ; Hansen, John A. / Increased apoptosis of peripheral blood T cells following allogeneic hematopoietic cell transplantation. :: Blood. 2000 ; 巻 95, 番号 12. pp. 3832-3839.
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title = "Increased apoptosis of peripheral blood T cells following allogeneic hematopoietic cell transplantation",
abstract = "Lymphopenia and immune deficiency are significant problems following allogeneic hematopoietic cell transplantation (HCT). It is largely assumed that delayed immune reconstruction is due to a profound decrease in thymus- dependent lymphopoiesis, especially in older patients, but apoptosis is also known to play a significant role in lymphocyte homeostasis. Peripheral T cells from patients who received HCT were studied for evidence of increased cell death. Spontaneous apoptosis was measured in CD3+ T cells following a 24-hour incubation using 7-amino-actinomycin D in conjunction with the dual staining of cell surface antigens. Apoptosis was significantly greater among CD3+ T cells taken from patients 19-23 days after transplantation (30.4{\%} ± 12.5{\%}, P < .05), and 1 year after transplantation (9.7{\%} ± 2.8{\%}, P < .05) compared with healthy controls (4.0{\%} ± 1.5{\%}). Increased apoptosis occurred preferentially in HLA (human leukocyte antigen)-DR positive cells and in both CD3+/CD4+ and CD3+/CD8+ T-cell subsets, while CD56+/CD3- natural killer cells were relatively resistant to apoptosis. The extent of CD4+ T-cell apoptosis was greater in patients with grade II-IV acute graft-versus-host disease (GVHD) (33.9{\%} ± 11.3{\%}) compared with grade 0-1 GVHD (14.6 ± 6.5{\%}, P < .05). T-cell apoptosis was also greater in patients who received transplantations from HLA-mismatched donors (39.5{\%} ± 10.4{\%}, P < .05) or HLA- matched unrelated donors (32.1{\%} ± 11.4{\%}, P < .05) compared with patients who received transplantations from HLA-identical siblings (19.6{\%} ± 6.7{\%}). The intensity of apoptosis among CD4+ T cells was significantly correlated with a lower CD4+ T-cell count. Together, these observations suggest that activation of T cells in vivo, presumably by alloantigens, predisposes the cells to spontaneous apoptosis, and this phenomenon is associated with lymphopenla. Activation-induced T-cell apoptosis may contribute to delayed immune reconstitution following HCT. (C) 2000 by The American Society of Hematology.",
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Lin, MT, Tseng, LH, Frangoul, H, Gooley, T, Pei, J, Barsoukov, A, Akatsuka, Y & Hansen, JA 2000, 'Increased apoptosis of peripheral blood T cells following allogeneic hematopoietic cell transplantation', Blood, 巻. 95, 番号 12, pp. 3832-3839.

Increased apoptosis of peripheral blood T cells following allogeneic hematopoietic cell transplantation. / Lin, Ming Tseh; Tseng, Li Hui; Frangoul, Haydar; Gooley, Ted; Pei, Ji; Barsoukov, Alexandre; Akatsuka, Yoshiki; Hansen, John A.

:: Blood, 巻 95, 番号 12, 15.06.2000, p. 3832-3839.

研究成果: Article

TY - JOUR

T1 - Increased apoptosis of peripheral blood T cells following allogeneic hematopoietic cell transplantation

AU - Lin, Ming Tseh

AU - Tseng, Li Hui

AU - Frangoul, Haydar

AU - Gooley, Ted

AU - Pei, Ji

AU - Barsoukov, Alexandre

AU - Akatsuka, Yoshiki

AU - Hansen, John A.

PY - 2000/6/15

Y1 - 2000/6/15

N2 - Lymphopenia and immune deficiency are significant problems following allogeneic hematopoietic cell transplantation (HCT). It is largely assumed that delayed immune reconstruction is due to a profound decrease in thymus- dependent lymphopoiesis, especially in older patients, but apoptosis is also known to play a significant role in lymphocyte homeostasis. Peripheral T cells from patients who received HCT were studied for evidence of increased cell death. Spontaneous apoptosis was measured in CD3+ T cells following a 24-hour incubation using 7-amino-actinomycin D in conjunction with the dual staining of cell surface antigens. Apoptosis was significantly greater among CD3+ T cells taken from patients 19-23 days after transplantation (30.4% ± 12.5%, P < .05), and 1 year after transplantation (9.7% ± 2.8%, P < .05) compared with healthy controls (4.0% ± 1.5%). Increased apoptosis occurred preferentially in HLA (human leukocyte antigen)-DR positive cells and in both CD3+/CD4+ and CD3+/CD8+ T-cell subsets, while CD56+/CD3- natural killer cells were relatively resistant to apoptosis. The extent of CD4+ T-cell apoptosis was greater in patients with grade II-IV acute graft-versus-host disease (GVHD) (33.9% ± 11.3%) compared with grade 0-1 GVHD (14.6 ± 6.5%, P < .05). T-cell apoptosis was also greater in patients who received transplantations from HLA-mismatched donors (39.5% ± 10.4%, P < .05) or HLA- matched unrelated donors (32.1% ± 11.4%, P < .05) compared with patients who received transplantations from HLA-identical siblings (19.6% ± 6.7%). The intensity of apoptosis among CD4+ T cells was significantly correlated with a lower CD4+ T-cell count. Together, these observations suggest that activation of T cells in vivo, presumably by alloantigens, predisposes the cells to spontaneous apoptosis, and this phenomenon is associated with lymphopenla. Activation-induced T-cell apoptosis may contribute to delayed immune reconstitution following HCT. (C) 2000 by The American Society of Hematology.

AB - Lymphopenia and immune deficiency are significant problems following allogeneic hematopoietic cell transplantation (HCT). It is largely assumed that delayed immune reconstruction is due to a profound decrease in thymus- dependent lymphopoiesis, especially in older patients, but apoptosis is also known to play a significant role in lymphocyte homeostasis. Peripheral T cells from patients who received HCT were studied for evidence of increased cell death. Spontaneous apoptosis was measured in CD3+ T cells following a 24-hour incubation using 7-amino-actinomycin D in conjunction with the dual staining of cell surface antigens. Apoptosis was significantly greater among CD3+ T cells taken from patients 19-23 days after transplantation (30.4% ± 12.5%, P < .05), and 1 year after transplantation (9.7% ± 2.8%, P < .05) compared with healthy controls (4.0% ± 1.5%). Increased apoptosis occurred preferentially in HLA (human leukocyte antigen)-DR positive cells and in both CD3+/CD4+ and CD3+/CD8+ T-cell subsets, while CD56+/CD3- natural killer cells were relatively resistant to apoptosis. The extent of CD4+ T-cell apoptosis was greater in patients with grade II-IV acute graft-versus-host disease (GVHD) (33.9% ± 11.3%) compared with grade 0-1 GVHD (14.6 ± 6.5%, P < .05). T-cell apoptosis was also greater in patients who received transplantations from HLA-mismatched donors (39.5% ± 10.4%, P < .05) or HLA- matched unrelated donors (32.1% ± 11.4%, P < .05) compared with patients who received transplantations from HLA-identical siblings (19.6% ± 6.7%). The intensity of apoptosis among CD4+ T cells was significantly correlated with a lower CD4+ T-cell count. Together, these observations suggest that activation of T cells in vivo, presumably by alloantigens, predisposes the cells to spontaneous apoptosis, and this phenomenon is associated with lymphopenla. Activation-induced T-cell apoptosis may contribute to delayed immune reconstitution following HCT. (C) 2000 by The American Society of Hematology.

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Lin MT, Tseng LH, Frangoul H, Gooley T, Pei J, Barsoukov A その他. Increased apoptosis of peripheral blood T cells following allogeneic hematopoietic cell transplantation. Blood. 2000 6 15;95(12):3832-3839.