TY - JOUR
T1 - Increased T-cell responses to Epstein-Barr virus with high viral load in patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma
AU - Morishima, Satoko
AU - Nakamura, Shigeo
AU - Yamamoto, Kazuhito
AU - Miyauchi, Hidemasa
AU - Kagami, Yoshitoyo
AU - Kinoshita, Tomohiro
AU - Onoda, Hiroshi
AU - Yatabe, Yasushi
AU - Ito, Masafumi
AU - Miyamura, Kouichi
AU - Nagai, Hirokazu
AU - Moritani, Suzuko
AU - Sugiura, Isamu
AU - Tsushita, Keitaro
AU - Mihara, Hidetsugu
AU - Ohbayashi, Kaneyuki
AU - Iba, Sachiko
AU - Emi, Nobuhiko
AU - Okamoto, Masataka
AU - Iwata, Seiko
AU - Kimura, Hiroshi
AU - Kuzushima, Kiyotaka
AU - Morishima, Yasuo
N1 - Publisher Copyright:
© 2014 Informa UK, Ltd.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Abstract The immunological status of patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma (EBV+ DLBCL) without obvious immunodeficiency has not been elucidated. A multicenter prospective study was conducted to assess pretreatment T-cell responses to EBV, EBV-DNA load and anti-EBV antibody in these patients. The proliferative and interferon (IFN)-γ-producing capacity of T-cells in response to autologous B-lymphoblastoid cell lines was determined using carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay. Frequencies of EBV-specific CD4+ T-cells in patients with EBV+ DLBCL (n = 13) were significantly higher than in healthy controls (HCs) (n = 16) after both ex vivo and in vitro stimulation. Frequencies of EBV-specific CD8+ T-cells in patients with EBV+ DLBCL tended to be higher than in HCs after in vitro stimulation. Patients with EBV+ DLBCL also showed increased immunoglobulin G (IgG) responses to lytic EBV-encoded antigens. Pretreatment plasma EBV-DNA level was significantly higher in patients with EBV+ DLBCL than in patients with EBV- DLBCL or HCs. In conclusion, EBV-specific T-cells showed increased reactivity, accompanied by higher levels of plasma virus DNA in patients with EBV+ DLBCL.
AB - Abstract The immunological status of patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma (EBV+ DLBCL) without obvious immunodeficiency has not been elucidated. A multicenter prospective study was conducted to assess pretreatment T-cell responses to EBV, EBV-DNA load and anti-EBV antibody in these patients. The proliferative and interferon (IFN)-γ-producing capacity of T-cells in response to autologous B-lymphoblastoid cell lines was determined using carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay. Frequencies of EBV-specific CD4+ T-cells in patients with EBV+ DLBCL (n = 13) were significantly higher than in healthy controls (HCs) (n = 16) after both ex vivo and in vitro stimulation. Frequencies of EBV-specific CD8+ T-cells in patients with EBV+ DLBCL tended to be higher than in HCs after in vitro stimulation. Patients with EBV+ DLBCL also showed increased immunoglobulin G (IgG) responses to lytic EBV-encoded antigens. Pretreatment plasma EBV-DNA level was significantly higher in patients with EBV+ DLBCL than in patients with EBV- DLBCL or HCs. In conclusion, EBV-specific T-cells showed increased reactivity, accompanied by higher levels of plasma virus DNA in patients with EBV+ DLBCL.
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U2 - 10.3109/10428194.2014.938326
DO - 10.3109/10428194.2014.938326
M3 - Article
C2 - 24975317
AN - SCOPUS:84929087777
SN - 1042-8194
VL - 56
SP - 1072
EP - 1078
JO - Leukemia and Lymphoma
JF - Leukemia and Lymphoma
IS - 4
ER -
