TY - JOUR
T1 - IncX2 and IncX1-X2 hybrid plasmids coexisting in a FosA6-producing Escherichia coli strain
AU - Guo, Qinglan
AU - Su, Jiachun
AU - McElheny, Christi Lee
AU - Stoesser, Nicole
AU - Doi, Yohei
AU - Wang, Minggui
N1 - Funding Information:
Q.G. was supported by grant numbers 81673479 and 81120108024 from the National Natural Science Foundation of China and grant number 16PJD010 from the Shanghai Pujiang Program. N.S. is funded by a University of Oxford Clinical Lectureship. Part of the analysis was supported by a research grant from the National Institutes of Health (R21AI123747). The funders had no role in study design, analysis, or preparation of the manuscript. Y.D. has served on advisory boards for Achaogen, Allergan, Curetis, The Medicines Company, and Roche and received research funding from The Medicines Company for studies unrelated to this work. No other authors have potential conflicts of interest.
PY - 2017/7
Y1 - 2017/7
N2 - IncX plasmids are receiving much attention as vehicles of carbapenem and colistin resistance genes, such as blaNDM, blaKPC, and mcr-1. Among them, IncX2 subgroup plasmids remain rare. Here, we characterized IncX2 and IncX1-X2 hybrid plasmids coexisting in a FosA6-producing Escherichia coli strain that were possibly generated as a consequence of recombination events between an R6K-like IncX2 plasmid and a pLN126_33-like IncX1 plasmid. Variable multidrug resistance mosaic regions were observed in these plasmids, indicating their potential to serve as flexible carriers of resistance genes. The diversity of IncX group plasmid backbones and accessory genes and the evolution of hybrid IncX plasmids pose a challenge in detecting and classifying them.
AB - IncX plasmids are receiving much attention as vehicles of carbapenem and colistin resistance genes, such as blaNDM, blaKPC, and mcr-1. Among them, IncX2 subgroup plasmids remain rare. Here, we characterized IncX2 and IncX1-X2 hybrid plasmids coexisting in a FosA6-producing Escherichia coli strain that were possibly generated as a consequence of recombination events between an R6K-like IncX2 plasmid and a pLN126_33-like IncX1 plasmid. Variable multidrug resistance mosaic regions were observed in these plasmids, indicating their potential to serve as flexible carriers of resistance genes. The diversity of IncX group plasmid backbones and accessory genes and the evolution of hybrid IncX plasmids pose a challenge in detecting and classifying them.
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U2 - 10.1128/AAC.00536-17
DO - 10.1128/AAC.00536-17
M3 - Article
C2 - 28438937
AN - SCOPUS:85021683396
VL - 61
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
SN - 0066-4804
IS - 7
M1 - e00536-17
ER -