Induced pluripotent stem cell-derived melanocyte precursor cells undergoing differentiation into melanocytes

Chieko Hosaka, Makoto Kunisada, Michiyo Koyanagi-Aoi, Taro Masaki, Chihiro Takemori, Mariko Ikeda, Takashi Aoi, Chikako Nishigori

研究成果: Article

抄録

Induced pluripotent stem cell (iPSC) technology offers a novel approach for conversion of human primary fibroblasts into melanocytes. During attempts to explore various protocols for differentiation of iPSCs into melanocytes, we found a distinct and self-renewing cell lineage that could differentiate into melanocytes, named as melanocyte precursor cells (MPCs). The MPCs exhibited a morphology distinctive from that of melanocytes, in lacking either the melanosomal structure or the melanocyte-specific marker genes MITF, TYR, and SOX10. In addition, gene expression studies in the MPCs showed high-level expression of WNT5A, ROR2, which are non-canonical WNT pathway markers, and its related receptor TGFβR2. In contrast, MPC differentiation into melanocytes was achieved by activating the canonical WNT pathway using the GSK3β inhibitor. Our data demonstrated the distinct characteristic of MPCs' ability to differentiate into melanocytes, and the underlying mechanism of interfacing between canonical WNT signaling pathway and non-canonical WNT signaling pathway.

元の言語English
ページ(範囲)623-633
ページ数11
ジャーナルPigment Cell and Melanoma Research
32
発行部数5
DOI
出版物ステータスPublished - 01-01-2019

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Induced Pluripotent Stem Cells
Melanocytes
Stem cells
Cell Differentiation
Fibroblasts
Gene expression
Genes
Cell Lineage

All Science Journal Classification (ASJC) codes

  • Oncology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Dermatology

これを引用

Hosaka, Chieko ; Kunisada, Makoto ; Koyanagi-Aoi, Michiyo ; Masaki, Taro ; Takemori, Chihiro ; Ikeda, Mariko ; Aoi, Takashi ; Nishigori, Chikako. / Induced pluripotent stem cell-derived melanocyte precursor cells undergoing differentiation into melanocytes. :: Pigment Cell and Melanoma Research. 2019 ; 巻 32, 番号 5. pp. 623-633.
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abstract = "Induced pluripotent stem cell (iPSC) technology offers a novel approach for conversion of human primary fibroblasts into melanocytes. During attempts to explore various protocols for differentiation of iPSCs into melanocytes, we found a distinct and self-renewing cell lineage that could differentiate into melanocytes, named as melanocyte precursor cells (MPCs). The MPCs exhibited a morphology distinctive from that of melanocytes, in lacking either the melanosomal structure or the melanocyte-specific marker genes MITF, TYR, and SOX10. In addition, gene expression studies in the MPCs showed high-level expression of WNT5A, ROR2, which are non-canonical WNT pathway markers, and its related receptor TGFβR2. In contrast, MPC differentiation into melanocytes was achieved by activating the canonical WNT pathway using the GSK3β inhibitor. Our data demonstrated the distinct characteristic of MPCs' ability to differentiate into melanocytes, and the underlying mechanism of interfacing between canonical WNT signaling pathway and non-canonical WNT signaling pathway.",
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Hosaka, C, Kunisada, M, Koyanagi-Aoi, M, Masaki, T, Takemori, C, Ikeda, M, Aoi, T & Nishigori, C 2019, 'Induced pluripotent stem cell-derived melanocyte precursor cells undergoing differentiation into melanocytes', Pigment Cell and Melanoma Research, 巻. 32, 番号 5, pp. 623-633. https://doi.org/10.1111/pcmr.12779

Induced pluripotent stem cell-derived melanocyte precursor cells undergoing differentiation into melanocytes. / Hosaka, Chieko; Kunisada, Makoto; Koyanagi-Aoi, Michiyo; Masaki, Taro; Takemori, Chihiro; Ikeda, Mariko; Aoi, Takashi; Nishigori, Chikako.

:: Pigment Cell and Melanoma Research, 巻 32, 番号 5, 01.01.2019, p. 623-633.

研究成果: Article

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