Induction of antitumor immunity by transduction of CD40 ligand gene and interferon-γ gene into lung cancer

Masahiro Noguchi, Kazuyoshi Imaizumi, Tsutomu Kawabe, Hisashi Wakayama, Yoshitsugu Horio, Yoshitaka Sekido, Toru Hara, Naozumi Hashimoto, Masahide Takahashi, Kaoru Shimokata, Yoshinori Hasegawa

研究成果: Article査読

29 被引用数 (Scopus)

抄録

CD40-CD40 ligand (CD40L) interaction is an important costimulatory signaling pathway in the crosstalk between T cells and antigen-presenting cells. This receptor-ligand system is known to be essential in eliciting strong cellular immunity. Here we demonstrate that murine lung cancer cells (3LLSA) transduced with the CD40L gene (3LLSA-CD40L) were rejected in syngeneic C57BL/6 mice, but grew in CD40-deficient mice to the same extent as control tumor cells. Immunohistochemical study showed that inflammatory cells, including CD4+, CD8+ T cells and NK cells, infiltrated into the inoculated 3LLSA-CD40L tumor tissue. Inoculation of 3LLSA-CD40L cells into mice resulted in the induction of 3LLSA-specific cytotoxic T-cell immunity, and the growth of parental 3LLSA tumors was inhibited when 3LLSA cells were inoculated into C57BL/6 mice mixed with 3LLSA-CD40L cells or when they were rechallenged 4 weeks after 3LLSA-CD40L cells were rejected. Furthermore, co-inoculation of interferon (IFN)-γ-transduced cells (3LLSA-IFNγ) with 3LLSA-CD40L cells enhanced the antitumor immunity efficiently in vivo. These results indicate that the in vivo priming with CD40L- and IFN-γ gene-transduced lung cancer cells is a promising strategy for inducing antitumor immunity in the treatment of lung cancer.

本文言語English
ページ(範囲)421-429
ページ数9
ジャーナルCancer Gene Therapy
8
6
DOI
出版ステータスPublished - 2001
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分子医療
  • 分子生物学
  • 癌研究

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