Induction of neuronal axon outgrowth by Shati/Nat8l by energy metabolism in mice cultured neurons

Kazuyuki Sumi, Kyosuke Uno, Shohei Matsumura, Yoshiaki Miyamoto, Yoko Furukawa-Hibi, Shin Ichi Muramatsu, Toshitaka Nabeshima, Atsumi Nitta

研究成果: Article

6 引用 (Scopus)

抜粋

A novel N-acetyltransferase, Shati/Nat8l, was identified in the nucleus accumbens of mice repeatedly treated with methamphetamine (METH). Shati/Nat8l has been reported to inhibit the pharmacological action induced by METH. Shati/Nat8l produces N-acetylaspartate from aspartate and acetyl-CoA. Previously, we reported that overexpression of Shati/Nat8l in nucleus accumbens attenuates the response to METH by N-acetylaspartylglutamate (which is derived from N-acetylaspartate)-mGluR3 signaling in the mice brain. In the present study, to clarify the type of cells that produce Shati/Nat8l, we carried out in-situ hybridization for the detection of Shati/Nat8l mRNA along with immunohistochemical studies using serial sections of mice brain. Shati/Nat8l mRNA was detected in neuronal cells, but not in astrocytes or microglia cells. Next, we investigated the function of Shati/Nat8l in the neuronal cells in mice brain; then, we used an adeno-associated virus vector containing Shati/Nat8l for transfection and overexpression of Shati/Nat8l protein into the primary cultured neurons to investigate the contribution toward the neuronal activity of Shati/Nat8l. Overexpression of Shati/Nat8l in the mice primary cultured neurons induced axonal growth, but not dendrite elongation at day 1.5 (DIV). This finding indicated that Shati/Nat8l contributes toward neuronal development. LY341495, a selective group II mGluRs antagonist, did not abolish this axonal growth, and N-acetylaspartylglutamate itself did not abolish axon outgrowth in the same cultured system. The cultured neurons overexpressing Shati/Nat8l contained high ATP, suggesting that axon outgrowth is dependent on energy metabolism. This study shows that Shati/Nat8l in the neuron may induce axon outgrowth by ATP synthesis and not through mGluR3 signaling.

元の言語English
ページ(範囲)740-746
ページ数7
ジャーナルNeuroreport
26
発行部数13
DOI
出版物ステータスPublished - 19-08-2015

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

これを引用

Sumi, K., Uno, K., Matsumura, S., Miyamoto, Y., Furukawa-Hibi, Y., Muramatsu, S. I., Nabeshima, T., & Nitta, A. (2015). Induction of neuronal axon outgrowth by Shati/Nat8l by energy metabolism in mice cultured neurons. Neuroreport, 26(13), 740-746. https://doi.org/10.1097/WNR.0000000000000416