Induction of tyrosine phosphorylation in human B lineage cells by crosslinking MB-1 molecule of B cell receptor-related heterodimer complex

Kazuhiko Kuwahara, Hideya Igarashi, Taro Kawai, Yasuhisa Ichigi, Atsushi Muraguchi, David Y. Mason, Masao Kimoto, Seiji Inui, Nobuo Sakaguchi

研究成果: Article査読

10 被引用数 (Scopus)

抄録

B cell antigen receptor is composed of immunoglobulin and associated MB-1 and B29. Here, we found that anti-human MB-1 stimulation induced tyrosine phosphorylation in immature B cells (FL4.4 and Nalm-6) but not in mature B cells (Daudi). Coprecipitated complex with the heterodimer component in Daudi and Nalm-6 contained the kinase molecule(s) which act on the heterodimer protein, while the complex in early lymphoid cell with germ line antigen receptor genes (FL4.4) did not. Candidate Fyn and Lyn are expressed in Nalm-6 and Daudi but are not expressed in FL4.4. These results suggested that src-type tyrosine kinases as Fyn and Lyn are responsible for the phosphorylation of MB-1 and B29 heterodimer, but anti-MB-1 stimulation can induce tyrosine phosphorylation reaction mediated by other kinase molecule(s) in the progenitor type cells.

本文言語English
ページ(範囲)1563-1569
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
197
3
DOI
出版ステータスPublished - 30-12-1993
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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