Inhibition by the cold receptor agonists menthol and icilin of airway smooth muscle contraction

Satoru Ito, Hiroaki Kume, Akira Shiraki, Masashi Kondo, Yasushi Makino, Kaichiro Kamiya, Yoshinori Hasegawa

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Menthol, known as a cold receptor agonist, has widely been used in the relief of respiratory symptoms such as coughing and chest congestion. Previous studies have demonstrated that menthol reduces bronchoconstriction and airway hyperresponsiveness. The aim of this study was to examine the effects of menthol and icilin, another cold receptor agonist, on airway smooth muscle contraction. Isometric force was monitored using epithelium-denuded tracheal smooth muscle tissues isolated from guinea pigs. Intracellular Ca2+ concentrations were assessed by fura-2 fluorescence. (-)Menthol (0.01-1 mM) inhibited contraction induced by methacholine (MCh, 0.01-10 μM) and high extracellular K+ concentrations (20-60 mM) in a concentration-dependent manner. Moreover, the increases of intracellular Ca2+ concentrations induced by MCh or high K+ were significantly reduced by (-)menthol. Icilin (100 μM) also significantly attenuated contraction induced by MCh or high K+. The inhibitory effect of 1 mM (-)menthol on MCh-induced contraction was significantly higher at cool temperature (24-26 °C) than at 37 °C. The present results demonstrate that inhibition of Ca2+ influx plays an important role in the menthol-mediated inhibition of contraction in airway smooth muscle. Furthermore, our findings indicate that stimulation of unknown cold receptors may be involved in these mechanisms. These findings suggest that the use of menthol is beneficial for reducing respiratory symptoms because of its inhibitory effects on airway smooth muscle contraction.

本文言語English
ページ(範囲)812-817
ページ数6
ジャーナルPulmonary Pharmacology and Therapeutics
21
5
DOI
出版ステータスPublished - 10-2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine
  • Biochemistry, medical
  • Pharmacology (medical)

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