TY - JOUR
T1 - Inhibitory action of guanosine 3′,5′-monophosphate on thrombin-induced phosphatidylinositol turnover and protein phosphorylation in human platelets
AU - Takai, Yoshimi
AU - Kaibuchi, Kozo
AU - Matsubara, Tsukasa
AU - Nishizuka, Yasutomi
PY - 1981/7/16
Y1 - 1981/7/16
N2 - Human platelets activated by thrombin rapidly produce diglyceride from phosphatidylinositol (PI). Concomitantly, a protein having a molecular weight of about 40,000 is heavily phosphorylated and serotonin is released. These reactions are inhibited in parallel manners by sodium nitroprusside which elevates cyclic GMP. 8-Bromocyclic GMP also inhibits the reactions. Instead, both agents stimulate the phosphorylation of a distinct protein having a molecular weight of about 50,000. Since stimulants of platelets such as thrombin normally increase cyclic GMP, the results imply that this cyclic nucleotide may be involved in a circuit leading to the feedback inhibition of PI hydrolysis presumably through protein phosphorylation.
AB - Human platelets activated by thrombin rapidly produce diglyceride from phosphatidylinositol (PI). Concomitantly, a protein having a molecular weight of about 40,000 is heavily phosphorylated and serotonin is released. These reactions are inhibited in parallel manners by sodium nitroprusside which elevates cyclic GMP. 8-Bromocyclic GMP also inhibits the reactions. Instead, both agents stimulate the phosphorylation of a distinct protein having a molecular weight of about 50,000. Since stimulants of platelets such as thrombin normally increase cyclic GMP, the results imply that this cyclic nucleotide may be involved in a circuit leading to the feedback inhibition of PI hydrolysis presumably through protein phosphorylation.
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U2 - 10.1016/S0006-291X(81)80010-1
DO - 10.1016/S0006-291X(81)80010-1
M3 - Article
C2 - 6269550
AN - SCOPUS:0019792855
SN - 0006-291X
VL - 101
SP - 61
EP - 67
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -