Integration of humoral and cellular HLA-specific immune responses in cord blood allograft rejection

R. Hanajiri, M. Murata, K. Sugimoto, M. Murase, R. Sakemura, T. Goto, K. Watanabe, N. Imahashi, S. Terakura, H. Ohashi, Y. Akatsuka, S. Kurahashi, K. Miyamura, H. Kiyoi, T. Nishida, T. Naoe

研究成果: ジャーナルへの寄稿学術論文査読

8 被引用数 (Scopus)

抄録

In allo-stem cell transplantation (SCT), it is unclear whether donor-specific anti-HLA Abs (DSAs) can actually mediate graft rejection or if they are simply surrogate markers for the cellular immunity that causes graft rejection. Here, we first analyzed a case of cord blood allograft rejection in which DSA and cytotoxic T lymphocyte (CTL) specific for donor HLA-B∗54:01 were detected at the time of graft rejection. Both the DSA and CTL inhibited colony formation by unrelated bone marrow mononuclear cells sharing HLA-B∗54:01, suggesting that the humoral and cellular immune responses were involved in the graft rejection. Interestingly, the DSA and CTL were also detected in cryopreserved pre-transplant patient blood, raising a hypothesis that the presence of anti-HLA Abs could be an indicator for corresponding HLA-specific T cells. We then evaluated the existence of HLA-specific CD8 + T cells in other patient blood specimens having anti-HLA class I Abs. Interferon-γ enzyme-linked immunospot assays clearly confirmed the existence of corresponding HLA-specific T-cell precursors in three of seven patients with anti-HLA Abs. In conclusion, our data demonstrate that integrated humoral and cellular immunity recognizing the same alloantigen of the donor can mediate graft rejection in DSA-positive patients undergoing HLA-mismatched allo-SCT. Further studies generalizing our observation are warranted.

本文言語英語
ページ(範囲)1187-1194
ページ数8
ジャーナルBone Marrow Transplantation
50
9
DOI
出版ステータス出版済み - 04-09-2015

All Science Journal Classification (ASJC) codes

  • 血液学
  • 移植

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