Interaction of human T-cell lymphotropic virus type I Rex protein with Dicer suppresses RNAi silencing

Makoto Abe, Hitoshi Suzuki, Hironori Nishitsuji, Hisatoshi Shida, Hiroshi Takaku

研究成果: ジャーナルへの寄稿学術論文査読

30 被引用数 (Scopus)

抄録

Double-stranded RNAs suppress the expression of homologous genes through an evolutionarily conserved process called RNA interference (RNAi) or post-transcriptional gene silencing. A bidentate nuclease called Dicer has been implicated as the protein responsible for the production of short interfering RNAs (siRNAs). In our experiments, Rex overexpression reduced the efficiency of short hairpin RNA (shRNA)-mediated RNAi. The interaction of Dicer with Rex inhibited the conversion of shRNA to siRNA. These results suggest that the interaction of Dicer with HTLV-I Rex inhibits Dicer activity and thereby reduces the efficiency of the conversion of shRNA to siRNA. Structured summary: MINT-7996954: Rex (uniprotkb:. P0C205) physically interacts (MI:. 0915) with Dicer (uniprotkb:. Q9UPY3) by pull down (MI:. 0096). MINT-7996926: Rex (uniprotkb:. P0C205) and Dicer (uniprotkb:. Q9UPY3) colocalize (MI:. 0403) by fluorescence microscopy (MI:. 0416). MINT-7996905: Dicer (uniprotkb:. Q9UPY3) physically interacts (MI:. 0915) with Rex (uniprotkb:. P0C205) by anti bait coimmunoprecipitation (MI:. 0006). MINT-7996852, MINT-7996870, MINT-7996886, MINT-7996830: Rex (uniprotkb:. P0C205) physically interacts (MI:. 0915) with Dicer (uniprotkb:. Q9UPY3) by anti tag coimmunoprecipitation (MI:. 0007).

本文言語英語
ページ(範囲)4313-4318
ページ数6
ジャーナルFEBS Letters
584
20
DOI
出版ステータス出版済み - 2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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