TY - JOUR
T1 - Interaction of human T-cell lymphotropic virus type I Rex protein with Dicer suppresses RNAi silencing
AU - Abe, Makoto
AU - Suzuki, Hitoshi
AU - Nishitsuji, Hironori
AU - Shida, Hisatoshi
AU - Takaku, Hiroshi
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for High Technology Research (No. 09309011 ) from the Ministry of Education, Science, Sports, and Culture in Japan and by a Grant-in-Aid for AIDS research from the Ministry of Health, Labor, and Welfare, Japan .
PY - 2010
Y1 - 2010
N2 - Double-stranded RNAs suppress the expression of homologous genes through an evolutionarily conserved process called RNA interference (RNAi) or post-transcriptional gene silencing. A bidentate nuclease called Dicer has been implicated as the protein responsible for the production of short interfering RNAs (siRNAs). In our experiments, Rex overexpression reduced the efficiency of short hairpin RNA (shRNA)-mediated RNAi. The interaction of Dicer with Rex inhibited the conversion of shRNA to siRNA. These results suggest that the interaction of Dicer with HTLV-I Rex inhibits Dicer activity and thereby reduces the efficiency of the conversion of shRNA to siRNA. Structured summary: MINT-7996954: Rex (uniprotkb:. P0C205) physically interacts (MI:. 0915) with Dicer (uniprotkb:. Q9UPY3) by pull down (MI:. 0096). MINT-7996926: Rex (uniprotkb:. P0C205) and Dicer (uniprotkb:. Q9UPY3) colocalize (MI:. 0403) by fluorescence microscopy (MI:. 0416). MINT-7996905: Dicer (uniprotkb:. Q9UPY3) physically interacts (MI:. 0915) with Rex (uniprotkb:. P0C205) by anti bait coimmunoprecipitation (MI:. 0006). MINT-7996852, MINT-7996870, MINT-7996886, MINT-7996830: Rex (uniprotkb:. P0C205) physically interacts (MI:. 0915) with Dicer (uniprotkb:. Q9UPY3) by anti tag coimmunoprecipitation (MI:. 0007).
AB - Double-stranded RNAs suppress the expression of homologous genes through an evolutionarily conserved process called RNA interference (RNAi) or post-transcriptional gene silencing. A bidentate nuclease called Dicer has been implicated as the protein responsible for the production of short interfering RNAs (siRNAs). In our experiments, Rex overexpression reduced the efficiency of short hairpin RNA (shRNA)-mediated RNAi. The interaction of Dicer with Rex inhibited the conversion of shRNA to siRNA. These results suggest that the interaction of Dicer with HTLV-I Rex inhibits Dicer activity and thereby reduces the efficiency of the conversion of shRNA to siRNA. Structured summary: MINT-7996954: Rex (uniprotkb:. P0C205) physically interacts (MI:. 0915) with Dicer (uniprotkb:. Q9UPY3) by pull down (MI:. 0096). MINT-7996926: Rex (uniprotkb:. P0C205) and Dicer (uniprotkb:. Q9UPY3) colocalize (MI:. 0403) by fluorescence microscopy (MI:. 0416). MINT-7996905: Dicer (uniprotkb:. Q9UPY3) physically interacts (MI:. 0915) with Rex (uniprotkb:. P0C205) by anti bait coimmunoprecipitation (MI:. 0006). MINT-7996852, MINT-7996870, MINT-7996886, MINT-7996830: Rex (uniprotkb:. P0C205) physically interacts (MI:. 0915) with Dicer (uniprotkb:. Q9UPY3) by anti tag coimmunoprecipitation (MI:. 0007).
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U2 - 10.1016/j.febslet.2010.09.031
DO - 10.1016/j.febslet.2010.09.031
M3 - Article
C2 - 20869963
AN - SCOPUS:77957775164
VL - 584
SP - 4313
EP - 4318
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 20
ER -