Interaction of Sec4 with GDI proteins from bovine brain, Drosophila melanogaster and Saccharomyces cerevisiae. Conservation of GDI membrane dissociation activity

Michelle D. Garrett, Alisa K. Kabcenell, Joseph E. Zahner, Kozo Kaibuchi, Takuya Sasaki, Yoshimi Takai, Clarissa M. Cheney, Peter J. Novick

研究成果: ジャーナルへの寄稿学術論文査読

39 被引用数 (Scopus)

抄録

Rab GDP dissociation inhibitor (Rab GDI), will induce the dissociation of GDP-bound rab3A from synaptic membranes and will inhibit GDP dissociation from Sec4, a member of the Rab subgroup of the Ras GTPase superfamily which is required for exocytosis in Saccharomyces cerevisiae. We report that Rab GDI releases GDP-bound Sec4 from yeast membranes. dGDI, a Drosophila homologue can similarly inhibit GDP dissociation from Sec4 and release GDP-bound Sec4 from yeast membranes. An activity partially purified from yeast cytosol dissociates GDP-bound Sec4 from yeast membranes, suggesting that yeast also possess a GDI protein that functions to recycle Sec4 from its target membrane.

本文言語英語
ページ(範囲)233-238
ページ数6
ジャーナルFEBS Letters
331
3
DOI
出版ステータス出版済み - 04-10-1993
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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