Intercellular trafficking of herpes simplex virus type 2 UL14 deletion mutant proteins

Yohei Yamauchi, Fumi Goshima, Tetsushi Yoshikawa, Naoki Nozawa, Tetsuo Koshizuka, Yukihiro Nishiyama

研究成果: Article査読

6 被引用数 (Scopus)


The UL14 gene product of herpes simplex virus is a 32 kDa protein expressed late in infection and is a minor component of the virion tegument. We recently showed that the wild-type UL14 protein has heat shock protein (HSP)-like and/or molecular chaperone-like functions. In this study, the intracellular localization of UL14 wild-type and deletion mutant proteins was examined in transfected cells by immunofluorescence. We found that N-terminus deleted but not wild-type/C-terminus deleted mutant proteins showed a significant number of cytoplasmic, multi-cellular stains in transfected Vero cells. The effect was greatly intensified by subjecting cells to heat shock at 43°C, whereas it was obstructed by treatment with the microfilament-disrupting drug cytochalas in D. The staining patterns of UL14 antigen-positive cells after heat shock suggested a cell-to-cell spread of the protein. Although the mechanism is unclear, the phenomenon seems to be an unprecedented type of intercellular trafficking.

ジャーナルBiochemical and Biophysical Research Communications
出版ステータスPublished - 2002

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学


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