Intraneuronally injected amyloid beta inhibits long-term potentiation in rat hippocampal slices

Izumi Nomura, Hajime Takechi, Nobuo Kato

研究成果: Article査読

25 被引用数 (Scopus)

抄録

Extracellular accumulation of amyloid beta (Aβ) is a hallmark of Alzheimer's disease (AD). It has been reported that extracellular perfusion of Aβ inhibits long-term potentiation (LTP), which is strongly related to memory in animal models. However, it has recently been proposed that intracellular Aβ may be the first pathological change to occur in AD. Here, we have investigated the effect on LTP of intracellular injection of Aβ (Aβ1-40, Aβ1-42) into hippocampal pyramidal cells using patch-clamp technique. We found that injection of 1 nM Aβ1-42 completely blocked LTP, and extracellular perfusion of a p38 MAPK inhibitor or a metabotropic glutamate receptor blocker reversed these blocking effects on LTP. Furthermore, we have examined the effects of different concentrations of Aβ1-40 and Aβ1-42 on LTP and showed that Aβ1-40 required a 1,000-fold higher concentration to attenuate LTP than 1 nM Aβ1-42. These results indicate that LTP is impaired by Aβ injected into genetically wild-type neurons in the sliced hippocampus, suggesting an acute action of intracellular Aβ on the intracellular LTP-inducing machinery.

本文言語English
ページ(範囲)2526-2531
ページ数6
ジャーナルJournal of Neurophysiology
107
9
DOI
出版ステータスPublished - 01-05-2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology

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