TY - JOUR
T1 - Investigation of Anti-Hyaluronidase Treatment on Vocal Fold Wound Healing
AU - Rousseau, Bernard
AU - Tateya, Ichiro
AU - Lim, Xin Hong
AU - Munoz-del-Rio, Alejandro
AU - Bless, Diane M.
N1 - Funding Information:
Supported by NIH Grants R01 DC 4428 & F31 DC 006314 from the National Institute on Deafness and other Communication Disorders (NIDCD).
PY - 2006/9
Y1 - 2006/9
N2 - Summary: Phytochemical constituents of medicinal plants demonstrate inhibition of tissue and bacterial hyaluronidase. Echinacoside is a caffeoyl conjugate of Echinacea with known anti-hyaluronidase properties. The purpose of this study was to investigate the wound healing effects of Echinacea on vocal fold wound healing and functional voice outcomes. Pig animal model. Methods: Vocal fold injury was induced in 18 pigs by unilateral vocal fold stripping. The uninjured vocal fold served as control. Three groups of six pigs randomly received a topical application of 300, 600, or 1200 mg of standardized Echinacea on the injured side. Animals were euthanized after 3, 10, and 15 days of wound healing. Phonation threshold pressure and vocal economy measurements were obtained from excised larynges. Treatment outcomes were examined by comparing the animals receiving treatment with a set of 19 untreated and 5 historical controls. Treatment effects on wound healing were evaluated by histologic staining for hyaluronan and collagen. Treated larynges revealed improved vocal economy and phonation threshold pressure compared with untreated larynges. Histologically, treated vocal folds revealed stable hyaluronan content and no significant accumulation of collagen compared with control. Findings provide a favorable outcome of anti-hyaluronidase treatment on acute vocal fold wound healing and functional measures of voice.
AB - Summary: Phytochemical constituents of medicinal plants demonstrate inhibition of tissue and bacterial hyaluronidase. Echinacoside is a caffeoyl conjugate of Echinacea with known anti-hyaluronidase properties. The purpose of this study was to investigate the wound healing effects of Echinacea on vocal fold wound healing and functional voice outcomes. Pig animal model. Methods: Vocal fold injury was induced in 18 pigs by unilateral vocal fold stripping. The uninjured vocal fold served as control. Three groups of six pigs randomly received a topical application of 300, 600, or 1200 mg of standardized Echinacea on the injured side. Animals were euthanized after 3, 10, and 15 days of wound healing. Phonation threshold pressure and vocal economy measurements were obtained from excised larynges. Treatment outcomes were examined by comparing the animals receiving treatment with a set of 19 untreated and 5 historical controls. Treatment effects on wound healing were evaluated by histologic staining for hyaluronan and collagen. Treated larynges revealed improved vocal economy and phonation threshold pressure compared with untreated larynges. Histologically, treated vocal folds revealed stable hyaluronan content and no significant accumulation of collagen compared with control. Findings provide a favorable outcome of anti-hyaluronidase treatment on acute vocal fold wound healing and functional measures of voice.
UR - https://www.scopus.com/pages/publications/33747132906
UR - https://www.scopus.com/pages/publications/33747132906#tab=citedBy
U2 - 10.1016/j.jvoice.2005.06.002
DO - 10.1016/j.jvoice.2005.06.002
M3 - Article
C2 - 16243482
AN - SCOPUS:33747132906
SN - 0892-1997
VL - 20
SP - 443
EP - 451
JO - Journal of Voice
JF - Journal of Voice
IS - 3
ER -