TY - JOUR
T1 - Investigation of Ogikenchuto (TJ-98) for intestinal fibrosis using a mouse model of dextran sulfate sodium-induced colitis
AU - Watanabe, Shunsuke
AU - Inoue, Mikihiro
AU - Naoe, Atsuki
AU - Murayama, Mika
N1 - Publisher Copyright:
© 2023 Japan Society for Oriental Medicine and Japan Society of Medical and Pharmaceutical Sciences for Traditional Medicine.
PY - 2024/4
Y1 - 2024/4
N2 - Background: Ulcerative colitis (UC) is a chronic inflammatory disease of unknown etiology. Although various new drugs have been developed in recent years, many of them are expensive, generating a demand for inexpensive and useful therapeutic drugs. Ogikenchuto (TJ-98), an existing Kampo medicine, has been used to treat ulcers and similar conditions for some time. The current study therefore investigated whether TJ-98 could be a new therapeutic agent for UC, a chronic inflammatory disease. Methods: This study used 6-week-old female C57BL/6J mice to establish a mouse model of dextran sulfate sodium (DSS)-induced colitis. We then evaluated the therapeutic effects of tacrolimus and TJ-98 on colitis based on body weight, intestinal length, intestinal fibrosis, and cytokines. Sirius red staining was used to evaluate intestinal fibrosis, while interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) were used to evaluate cytokines involved in inflammation. Results: Neither tacrolimus nor TJ-98 ameliorated weight loss. Although tacrolimus did not remediate intestinal shortening, intestinal fibrosis, or cytokine levels (IL-1β, IL-6, and TNF-α), TJ-98 did ameliorate intestinal shortening and intestinal fibrosis and decrease IL-1β levels. Conclusions: This study confirmed that TJ-98 suppressed the inflammation caused by DSS-induced enteritis and decreased the associated intestinal fibrosis, highlighting its potential as an inexpensive novel therapeutic agent for UC.
AB - Background: Ulcerative colitis (UC) is a chronic inflammatory disease of unknown etiology. Although various new drugs have been developed in recent years, many of them are expensive, generating a demand for inexpensive and useful therapeutic drugs. Ogikenchuto (TJ-98), an existing Kampo medicine, has been used to treat ulcers and similar conditions for some time. The current study therefore investigated whether TJ-98 could be a new therapeutic agent for UC, a chronic inflammatory disease. Methods: This study used 6-week-old female C57BL/6J mice to establish a mouse model of dextran sulfate sodium (DSS)-induced colitis. We then evaluated the therapeutic effects of tacrolimus and TJ-98 on colitis based on body weight, intestinal length, intestinal fibrosis, and cytokines. Sirius red staining was used to evaluate intestinal fibrosis, while interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) were used to evaluate cytokines involved in inflammation. Results: Neither tacrolimus nor TJ-98 ameliorated weight loss. Although tacrolimus did not remediate intestinal shortening, intestinal fibrosis, or cytokine levels (IL-1β, IL-6, and TNF-α), TJ-98 did ameliorate intestinal shortening and intestinal fibrosis and decrease IL-1β levels. Conclusions: This study confirmed that TJ-98 suppressed the inflammation caused by DSS-induced enteritis and decreased the associated intestinal fibrosis, highlighting its potential as an inexpensive novel therapeutic agent for UC.
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U2 - 10.1002/tkm2.1391
DO - 10.1002/tkm2.1391
M3 - Article
AN - SCOPUS:85179333372
SN - 1880-1447
VL - 11
SP - 3
EP - 12
JO - Traditional and Kampo Medicine
JF - Traditional and Kampo Medicine
IS - 1
ER -