Involvement of AMPA receptor desensitization in short-term synaptic depression at the calyx of Held in developing rats

Maki Koike-Tani, Takeshi Kanda, Naoto Saitoh, Takayuki Yamashita, Tomoyuki Takahashi

研究成果: Article査読

40 被引用数 (Scopus)

抄録

Paired-pulse facilitation (PPF) and depression (PPD) are forms of short-term plasticity that are generally thought to reflect changes in transmitter release probability. However, desensitization of postsynaptic AMPA receptors (AMPARs) significantly contributes to PPD at many glutamatergic synapses. To clarify the involvement of AMPAR desensitization in synaptic PPD, we compared PPD with AMPAR desensitization, induced by paired-pulse glutamate application in patches excised from postsynaptic cells at the calyx of Held synapse of developing rats. We found that AMPAR desensitization contributed significantly to PPD before the onset of hearing (P10 - 12), but that its contribution became negligible after hearing onset. During postnatal development (P7 - 21) the recovery of AMPARs from desensitization became faster. Concomitantly, glutamate sensitivity of AMPAR desensitization declined. Single-cell reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated a developmental decline of GluR1 expression that correlated with speeding of the recovery of AMPARs from desensitization. Transmitter release probability declined during the second postnatal week (P7 - 14). Manipulation of the extracellular Ca2+/Mg2+ ratio, to match release probability at P7 - 8 and P13 - 15 synapses, revealed that the release probability is also an important factor determining the involvement of AMPAR desensitization in PPD. We conclude that the extent of involvement of AMPAR desensitization in short-term synaptic depression is determined by both pre- and postsynaptic mechanisms.

本文言語English
ページ(範囲)2263-2275
ページ数13
ジャーナルJournal of Physiology
586
9
DOI
出版ステータスPublished - 01-05-2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生理学

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