@article{56b054bc4a0f495d95b9c163c313e40b,
title = "Involvement of GANP in B cell activation in T cell-dependent antigen response",
abstract = "Adaptive immunity is dependent on proliferation of antigen-driven B cells for clonal expansion in germinal centers (GCs) against T cell-dependent antigens (TD-Ag), accompanied with somatic hypermutation of variable-region gene and class switching of B cell antigen receptors. To study molecular mechanisms for B cell differentiation in GCs, we have identified and studied a 210kDa GANP protein expressed in GC-B cells. GANP has domains for MCM3-binding and RNA-primase activities and is selectively up-regulated in centrocytes surrounded with follicular dendritic cells (FDCs) upon immunization with TD-Ag in vivo and in B cells stimulated with anti-CD40 monoclonal antibody in vitro, which suggested that GANP plays a certain important role in the maturation of immunoglobulin or selection of B cells in GC during the immune response to TD-Ag. Since this up-regulation has not been detected in T cells in GCs and in Concanavalin A-stimulated T cells in vitro, selective function of GANP molecule on B cell proliferation and differentiation might exist.",
keywords = "CD40, GCs, RNA-primase, TD-Ag",
author = "Nobuo Sakaguchi and Satoru Fujimura and Kazuhiko Kuwahara",
note = "Funding Information: Sakaguchi Nobuo 1, 2 Fujimura Satoru 1 Kuwahara Kazuhiko 1, 3 1 Department of Immunology Graduate School of Medical Sciences Kumamoto University 1-1-1 Honjo, Kumamoto 860-0811 Japan kumamoto-u.ac.jp 2 CREST Japan Science and Technology Corporation (JST) Japan jst.go.jp 3 PRESTO Japan Science and Technology Corporation (JST) Japan jst.go.jp 2002 9 3 169 172 2002 Copyright {\textcopyright} 2002 Hindawi Publishing Corporation Adaptive immunity is dependent on proliferation of antigen-driven B cells for clonal expansion in germinal centers (GCs) against T cell-dependent antigens (TD-Ag), accompanied with somatic hypermutation of variable-region gene and class switching of B cell antigen receptors. To study molecular mechanisms for B cell differentiation in GCs, we have identified and studied a 210 kDa GANP protein expressed in GC-B cells. GANP has domains for MCM3-binding and RNA-primase activities and is selectively up-regulated in centrocytes surrounded with follicular dendritic cells (FDCs) upon immunization with TD-Ag in vivo and in B cells stimulated with anti-CD40 monoclonal antibody in vitro , which suggested that GANP plays a certain important role in the maturation of immunoglobulin or selection of B cells in GC during the immune response to TD-Ag. Since this up-regulation has not been detected in T cells in GCs and in Concanavalin A-stimulated T cells in vitro , selective function of GANP molecule on B cell proliferation and differentiation might exist. http://dx.doi.org/10.13039/501100001700 Ministry of Education, Culture, Sports, Science, and Technology Uehara Memorial Foundation ",
year = "2002",
month = sep,
doi = "10.1080/1044667031000137647",
language = "English",
volume = "9",
pages = "169--172",
journal = "Clinical and Developmental Immunology",
issn = "1044-6672",
publisher = "Taylor and Francis Ltd.",
number = "3",
}