Involvement of heme oxygenase-1 in kaempferol-induced anti-allergic actions in RBL-2H3 cells

Etsuko Hirose; Miyoko Matsushima; Kenzo Takagi; Yui Ota; Keiko Ishigami; Tatsuya Hirayama; Yuta Hayashi; Toshinobu Nakamura; Naozumi Hashimoto; Kazuyoshi Imaizumi; Kenji Baba; Yoshinori Hasegawa; Tsutomu Kawabe

doi:10.1007/s10753-009-9108-4

Involvement of heme oxygenase-1 in kaempferol-induced anti-allergic actions in RBL-2H3 cells

Etsuko Hirose, Miyoko Matsushima, Kenzo Takagi, Yui Ota, Keiko Ishigami, Tatsuya Hirayama, Yuta Hayashi, Toshinobu Nakamura, Naozumi Hashimoto, Kazuyoshi Imaizumi, Kenji Baba, Yoshinori Hasegawa, Tsutomu Kawabe

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

22 被引用数 (Scopus)

抄録

Kaempferol is one of the most commonly found dietary flavonoids. The exposure to kaempferol is known to inhibit degranulation from mast cells, but the inhibitory mechanism of degranulation has not been clarified yet. In this study, we investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of kaempferol against degranulation in rat basophilic leukemia (RBL-2H3) cells. Our results demonstrate upregulation of HO enzymatic activity after short (15 min) exposure to kaempferol, followed by the induction of HO-1 expression in protein. The involvement of HO-1 in the kaempferol-induced inhibition of degranulation was confirmed using tin protoporphyrin IX (SnPP), a HO-1 inhibitor. These findings strongly suggest that kaempferol exerts anti-allergic actions via activation of the HO-1.

本文言語	英語
ページ（範囲）	99-108
ページ数	10
ジャーナル	Inflammation
巻	32
号	2
DOI	https://doi.org/10.1007/s10753-009-9108-4
出版ステータス	出版済み - 04-2009
外部発表	はい

All Science Journal Classification (ASJC) codes

免疫アレルギー学
免疫学

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10.1007/s10753-009-9108-4

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@article{6317e815faee49f3ab9bf44391f923f0,

title = "Involvement of heme oxygenase-1 in kaempferol-induced anti-allergic actions in RBL-2H3 cells",

abstract = "Kaempferol is one of the most commonly found dietary flavonoids. The exposure to kaempferol is known to inhibit degranulation from mast cells, but the inhibitory mechanism of degranulation has not been clarified yet. In this study, we investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of kaempferol against degranulation in rat basophilic leukemia (RBL-2H3) cells. Our results demonstrate upregulation of HO enzymatic activity after short (15 min) exposure to kaempferol, followed by the induction of HO-1 expression in protein. The involvement of HO-1 in the kaempferol-induced inhibition of degranulation was confirmed using tin protoporphyrin IX (SnPP), a HO-1 inhibitor. These findings strongly suggest that kaempferol exerts anti-allergic actions via activation of the HO-1.",

author = "Etsuko Hirose and Miyoko Matsushima and Kenzo Takagi and Yui Ota and Keiko Ishigami and Tatsuya Hirayama and Yuta Hayashi and Toshinobu Nakamura and Naozumi Hashimoto and Kazuyoshi Imaizumi and Kenji Baba and Yoshinori Hasegawa and Tsutomu Kawabe",

note = "Funding Information: This work was supported in part by the Grant-in-Aid for the Scientific Research from the Japan Society for the Promotion of Science, and Grant-in-Aid of Nagono Medical Foundation (Nagoya, Japan), and Ichihara International Scholarship Foundation (Nagoya, Japan).",

year = "2009",

month = apr,

doi = "10.1007/s10753-009-9108-4",

language = "English",

volume = "32",

pages = "99--108",

journal = "Inflammation",

issn = "0360-3997",

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T1 - Involvement of heme oxygenase-1 in kaempferol-induced anti-allergic actions in RBL-2H3 cells

AU - Hirose, Etsuko

AU - Matsushima, Miyoko

AU - Takagi, Kenzo

AU - Ota, Yui

AU - Ishigami, Keiko

AU - Hirayama, Tatsuya

AU - Hayashi, Yuta

AU - Nakamura, Toshinobu

AU - Hashimoto, Naozumi

AU - Imaizumi, Kazuyoshi

AU - Baba, Kenji

AU - Hasegawa, Yoshinori

AU - Kawabe, Tsutomu

N1 - Funding Information: This work was supported in part by the Grant-in-Aid for the Scientific Research from the Japan Society for the Promotion of Science, and Grant-in-Aid of Nagono Medical Foundation (Nagoya, Japan), and Ichihara International Scholarship Foundation (Nagoya, Japan).

PY - 2009/4

Y1 - 2009/4

N2 - Kaempferol is one of the most commonly found dietary flavonoids. The exposure to kaempferol is known to inhibit degranulation from mast cells, but the inhibitory mechanism of degranulation has not been clarified yet. In this study, we investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of kaempferol against degranulation in rat basophilic leukemia (RBL-2H3) cells. Our results demonstrate upregulation of HO enzymatic activity after short (15 min) exposure to kaempferol, followed by the induction of HO-1 expression in protein. The involvement of HO-1 in the kaempferol-induced inhibition of degranulation was confirmed using tin protoporphyrin IX (SnPP), a HO-1 inhibitor. These findings strongly suggest that kaempferol exerts anti-allergic actions via activation of the HO-1.

AB - Kaempferol is one of the most commonly found dietary flavonoids. The exposure to kaempferol is known to inhibit degranulation from mast cells, but the inhibitory mechanism of degranulation has not been clarified yet. In this study, we investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of kaempferol against degranulation in rat basophilic leukemia (RBL-2H3) cells. Our results demonstrate upregulation of HO enzymatic activity after short (15 min) exposure to kaempferol, followed by the induction of HO-1 expression in protein. The involvement of HO-1 in the kaempferol-induced inhibition of degranulation was confirmed using tin protoporphyrin IX (SnPP), a HO-1 inhibitor. These findings strongly suggest that kaempferol exerts anti-allergic actions via activation of the HO-1.

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Involvement of heme oxygenase-1 in kaempferol-induced anti-allergic actions in RBL-2H3 cells

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