TY - JOUR
T1 - Iris-derived induced pluripotent stem cells that express GFP in all somatic cells of mice and differentiate into functional retinal neurons
AU - Hiramatsu, Noriko
AU - Yamamoto, Naoki
AU - Ohkuma, Mahito
AU - Nagai, Noriaki
AU - Miyachi, Ei ichi
AU - Yamatsuta, Kumiko
AU - Imaizumi, Kazuyoshi
N1 - Funding Information:
We thank Aya Ito, Ayaka Yoshino, Kana Ochiai (Kanazawa Medical University), and Hiromi Yamashita, Chieko Nishikawa, Yuka Yokoi, Yu Kato (Fujita Health University) for their help with the experiments, Mari Seto for English editing and technical supports, and Editage (www.editage.com) for English language editing.
Publisher Copyright:
© 2022, The Author(s) under exclusive licence to The Japanese Society for Clinical Molecular Morphology.
PY - 2022/12
Y1 - 2022/12
N2 - When regenerated tissue is generated from induced pluripotent stem cells (iPSCs), it is necessary to track and identify the transplanted cells. Fluorescently-labeled iPSCs synthesize a fluorescent substance that is easily tracked. However, the expressed protein should not affect the original genome sequence or pluripotency. To solve this problem, we created a cell tool for basic research on iPSCs. Iris tissue-derived cells from GFP fluorescence-expressing mice (GFP-DBA/2 mice) were reprogrammed to generate GFP mouse iris-derived iPSCs (M-iris GFP iPSCs). M-iris GFP iPSCs expressed cell markers characteristic of iPSCs and showed pluripotency in differentiating into the three germ layers. In addition, when expressing GFP, the cells differentiated into functional recoverin- and calbindin-positive cells. Thus, this cell line will facilitate future studies on iPSCs.
AB - When regenerated tissue is generated from induced pluripotent stem cells (iPSCs), it is necessary to track and identify the transplanted cells. Fluorescently-labeled iPSCs synthesize a fluorescent substance that is easily tracked. However, the expressed protein should not affect the original genome sequence or pluripotency. To solve this problem, we created a cell tool for basic research on iPSCs. Iris tissue-derived cells from GFP fluorescence-expressing mice (GFP-DBA/2 mice) were reprogrammed to generate GFP mouse iris-derived iPSCs (M-iris GFP iPSCs). M-iris GFP iPSCs expressed cell markers characteristic of iPSCs and showed pluripotency in differentiating into the three germ layers. In addition, when expressing GFP, the cells differentiated into functional recoverin- and calbindin-positive cells. Thus, this cell line will facilitate future studies on iPSCs.
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U2 - 10.1007/s00795-022-00330-z
DO - 10.1007/s00795-022-00330-z
M3 - Article
C2 - 35932315
AN - SCOPUS:85135695411
VL - 55
SP - 292
EP - 303
JO - Medical Molecular Morphology
JF - Medical Molecular Morphology
SN - 1860-1480
IS - 4
ER -