Isolation and expression of a human SRY-related cDNA, hSOX20

Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Koji Taniguchi, Takuma Fujii, Akihiro Umezawa, Jun Ichi Hata, Sadakazu Aiso

研究成果: Article

15 引用 (Scopus)

抄録

SOX is a family of genes related to the testis-determining gene, SRY. We have isolated and sequenced an hSOX20 cDNA: from a cell line of human embryonic carcinoma. This cDNA contains an open reading frame (ORF) encoding 233 amino acids. The protein encompasses an SRY-type HMG box exhibiting strong homologies to those of mouse Sox15 and Sox16. Various adult and fetal tissues were tested for hSOX20 mRNA by Northern analysis. Its expression is restricted to the fetal testis and the size of the transcript is 1.5 knt. Electrophoretic mobility shift assay indicated that recombinant hSOX20 polypeptide is capable of binding to AACAAT sequence.

元の言語English
ページ(範囲)132-137
ページ数6
ジャーナルBiochimica et Biophysica Acta - Gene Structure and Expression
1396
発行部数2
DOI
出版物ステータスPublished - 09-03-1998

Fingerprint

Testis
Complementary DNA
Genes
Electrophoretic mobility
Electrophoretic Mobility Shift Assay
Open Reading Frames
Assays
Fetus
Cells
Tissue
Carcinoma
Amino Acids
Cell Line
Messenger RNA
Peptides
Proteins

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

これを引用

Hiraoka, Yoshiki ; Ogawa, Motoyuki ; Sakai, Yukinao ; Taniguchi, Koji ; Fujii, Takuma ; Umezawa, Akihiro ; Hata, Jun Ichi ; Aiso, Sadakazu. / Isolation and expression of a human SRY-related cDNA, hSOX20. :: Biochimica et Biophysica Acta - Gene Structure and Expression. 1998 ; 巻 1396, 番号 2. pp. 132-137.
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abstract = "SOX is a family of genes related to the testis-determining gene, SRY. We have isolated and sequenced an hSOX20 cDNA: from a cell line of human embryonic carcinoma. This cDNA contains an open reading frame (ORF) encoding 233 amino acids. The protein encompasses an SRY-type HMG box exhibiting strong homologies to those of mouse Sox15 and Sox16. Various adult and fetal tissues were tested for hSOX20 mRNA by Northern analysis. Its expression is restricted to the fetal testis and the size of the transcript is 1.5 knt. Electrophoretic mobility shift assay indicated that recombinant hSOX20 polypeptide is capable of binding to AACAAT sequence.",
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Hiraoka, Y, Ogawa, M, Sakai, Y, Taniguchi, K, Fujii, T, Umezawa, A, Hata, JI & Aiso, S 1998, 'Isolation and expression of a human SRY-related cDNA, hSOX20', Biochimica et Biophysica Acta - Gene Structure and Expression, 巻. 1396, 番号 2, pp. 132-137. https://doi.org/10.1016/S0167-4781(97)00186-3

Isolation and expression of a human SRY-related cDNA, hSOX20. / Hiraoka, Yoshiki; Ogawa, Motoyuki; Sakai, Yukinao; Taniguchi, Koji; Fujii, Takuma; Umezawa, Akihiro; Hata, Jun Ichi; Aiso, Sadakazu.

:: Biochimica et Biophysica Acta - Gene Structure and Expression, 巻 1396, 番号 2, 09.03.1998, p. 132-137.

研究成果: Article

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T1 - Isolation and expression of a human SRY-related cDNA, hSOX20

AU - Hiraoka, Yoshiki

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AU - Sakai, Yukinao

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AU - Fujii, Takuma

AU - Umezawa, Akihiro

AU - Hata, Jun Ichi

AU - Aiso, Sadakazu

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N2 - SOX is a family of genes related to the testis-determining gene, SRY. We have isolated and sequenced an hSOX20 cDNA: from a cell line of human embryonic carcinoma. This cDNA contains an open reading frame (ORF) encoding 233 amino acids. The protein encompasses an SRY-type HMG box exhibiting strong homologies to those of mouse Sox15 and Sox16. Various adult and fetal tissues were tested for hSOX20 mRNA by Northern analysis. Its expression is restricted to the fetal testis and the size of the transcript is 1.5 knt. Electrophoretic mobility shift assay indicated that recombinant hSOX20 polypeptide is capable of binding to AACAAT sequence.

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