Isolation and expression of a human SRY-related cDNA, hSOX20

Yoshiki Hiraoka; Motoyuki Ogawa; Yukinao Sakai; Koji Taniguchi; Takuma Fujii; Akihiro Umezawa; Jun Ichi Hata; Sadakazu Aiso

doi:10.1016/S0167-4781(97)00186-3

Isolation and expression of a human SRY-related cDNA, hSOX20

Yoshiki Hiraoka
, Motoyuki Ogawa
, Yukinao Sakai
, Koji Taniguchi
, Takuma Fujii
, Akihiro Umezawa
, Jun Ichi Hata
, Sadakazu Aiso

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

16 被引用数 (Scopus)

抄録

SOX is a family of genes related to the testis-determining gene, SRY. We have isolated and sequenced an hSOX20 cDNA: from a cell line of human embryonic carcinoma. This cDNA contains an open reading frame (ORF) encoding 233 amino acids. The protein encompasses an SRY-type HMG box exhibiting strong homologies to those of mouse Sox15 and Sox16. Various adult and fetal tissues were tested for hSOX20 mRNA by Northern analysis. Its expression is restricted to the fetal testis and the size of the transcript is 1.5 knt. Electrophoretic mobility shift assay indicated that recombinant hSOX20 polypeptide is capable of binding to AACAAT sequence.

本文言語	英語
ページ（範囲）	132-137
ページ数	6
ジャーナル	Biochimica et Biophysica Acta - Gene Structure and Expression
巻	1396
号	2
DOI	https://doi.org/10.1016/S0167-4781(97)00186-3
出版ステータス	出版済み - 09-03-1998
外部発表	はい

All Science Journal Classification (ASJC) codes

構造生物学
生物理学
生化学
遺伝学

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10.1016/S0167-4781(97)00186-3

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title = "Isolation and expression of a human SRY-related cDNA, hSOX20",

abstract = "SOX is a family of genes related to the testis-determining gene, SRY. We have isolated and sequenced an hSOX20 cDNA: from a cell line of human embryonic carcinoma. This cDNA contains an open reading frame (ORF) encoding 233 amino acids. The protein encompasses an SRY-type HMG box exhibiting strong homologies to those of mouse Sox15 and Sox16. Various adult and fetal tissues were tested for hSOX20 mRNA by Northern analysis. Its expression is restricted to the fetal testis and the size of the transcript is 1.5 knt. Electrophoretic mobility shift assay indicated that recombinant hSOX20 polypeptide is capable of binding to AACAAT sequence.",

keywords = "DNA binding, Embryonic carcinoma, Fetal testis, HMG box, SOX",

author = "Yoshiki Hiraoka and Motoyuki Ogawa and Yukinao Sakai and Koji Taniguchi and Takuma Fujii and Akihiro Umezawa and Hata, \{Jun Ichi\} and Sadakazu Aiso",

note = "Funding Information: This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Minstry of Education, Science and Culture to S. A. and Grants-in Aids from Ministry of Education, Science and Culture to S. A. and Y. H., and by Keio Gakuji Fukuzawa Memorial Funds for the Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.",

year = "1998",

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doi = "10.1016/S0167-4781(97)00186-3",

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T1 - Isolation and expression of a human SRY-related cDNA, hSOX20

AU - Hiraoka, Yoshiki

AU - Ogawa, Motoyuki

AU - Sakai, Yukinao

AU - Taniguchi, Koji

AU - Fujii, Takuma

AU - Umezawa, Akihiro

AU - Hata, Jun Ichi

AU - Aiso, Sadakazu

N1 - Funding Information: This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Minstry of Education, Science and Culture to S. A. and Grants-in Aids from Ministry of Education, Science and Culture to S. A. and Y. H., and by Keio Gakuji Fukuzawa Memorial Funds for the Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.

PY - 1998/3/9

Y1 - 1998/3/9

N2 - SOX is a family of genes related to the testis-determining gene, SRY. We have isolated and sequenced an hSOX20 cDNA: from a cell line of human embryonic carcinoma. This cDNA contains an open reading frame (ORF) encoding 233 amino acids. The protein encompasses an SRY-type HMG box exhibiting strong homologies to those of mouse Sox15 and Sox16. Various adult and fetal tissues were tested for hSOX20 mRNA by Northern analysis. Its expression is restricted to the fetal testis and the size of the transcript is 1.5 knt. Electrophoretic mobility shift assay indicated that recombinant hSOX20 polypeptide is capable of binding to AACAAT sequence.

AB - SOX is a family of genes related to the testis-determining gene, SRY. We have isolated and sequenced an hSOX20 cDNA: from a cell line of human embryonic carcinoma. This cDNA contains an open reading frame (ORF) encoding 233 amino acids. The protein encompasses an SRY-type HMG box exhibiting strong homologies to those of mouse Sox15 and Sox16. Various adult and fetal tissues were tested for hSOX20 mRNA by Northern analysis. Its expression is restricted to the fetal testis and the size of the transcript is 1.5 knt. Electrophoretic mobility shift assay indicated that recombinant hSOX20 polypeptide is capable of binding to AACAAT sequence.

KW - DNA binding

KW - Embryonic carcinoma

KW - Fetal testis

KW - HMG box

KW - SOX

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UR - https://www.scopus.com/pages/publications/0032498831#tab=citedBy

U2 - 10.1016/S0167-4781(97)00186-3

DO - 10.1016/S0167-4781(97)00186-3

M3 - Article

C2 - 9540826

AN - SCOPUS:0032498831

SN - 0167-4781

VL - 1396

SP - 132

EP - 137

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 2

ER -

Isolation and expression of a human SRY-related cDNA, hSOX20

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