Isolation of a human monoclonal antibody with strong neutralizing activity against diphtheria toxin

Mai Kakita; Tsuyoshi Takahashi; Takako Komiya; Yoshitaka Iba; Takao Tsuji; Yoshikazu Kurosawa; Motohide Takahashi

doi:10.1128/IAI.01731-05

Isolation of a human monoclonal antibody with strong neutralizing activity against diphtheria toxin

Mai Kakita
, Tsuyoshi Takahashi
, Takako Komiya
, Yoshitaka Iba
, Takao Tsuji
, Yoshikazu Kurosawa
, Motohide Takahashi

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

16 被引用数 (Scopus)

抄録

We isolated a human monoclonal antibody against diphtheria toxin (DT). It bound to fragment B with a binding activity (K_d) of 3.01 nM. The neutralizing activity assayed by the rabbit skin test was estimated to be 73,600 IU/g. This could be used as a therapeutic drug against DT in place of the traditional equine sera.

本文言語	英語
ページ（範囲）	3682-3683
ページ数	2
ジャーナル	Infection and Immunity
巻	74
号	6
DOI	https://doi.org/10.1128/IAI.01731-05
出版ステータス	出版済み - 06-2006
外部発表	はい

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

SDG 3 すべての人に健康と福祉を

All Science Journal Classification (ASJC) codes

寄生虫科
微生物学
免疫学
感染症

文献へのアクセス

10.1128/IAI.01731-05

他のファイルとリンク

引用スタイル

@article{c1d572299db94d50a45c6ae492d0b9cd,

title = "Isolation of a human monoclonal antibody with strong neutralizing activity against diphtheria toxin",

abstract = "We isolated a human monoclonal antibody against diphtheria toxin (DT). It bound to fragment B with a binding activity (Kd) of 3.01 nM. The neutralizing activity assayed by the rabbit skin test was estimated to be 73,600 IU/g. This could be used as a therapeutic drug against DT in place of the traditional equine sera.",

author = "Mai Kakita and Tsuyoshi Takahashi and Takako Komiya and Yoshitaka Iba and Takao Tsuji and Yoshikazu Kurosawa and Motohide Takahashi",

note = "Funding Information: The assistance of residents of the Tahuayo, Amazon, Ucayali, Putumayo, and Nanay Rivers is gratefully acknowledged. Special thanks go to colleagues and staff at IIAP-Iquitos, IIAP-Pucallpa, INIA-Iquitos, PNCC-Iquitos, PNCCPucallpa, INADE-Iquitos, INRENA-El Estrecho, and INPA-Manaus. Mario Pinedo, Jorge Soplin, Dennis Huayllahua and Pilar Diaz are acknowledged for their exceptional support and collaboration. Field research was generously funded by the Rainforest Conservation Fund (RCF) of Chicago, Illinois; the Dallas Zoological Society, and the Tropical Conservation and Development Program of the University of Florida.",

year = "2006",

month = jun,

doi = "10.1128/IAI.01731-05",

language = "English",

volume = "74",

pages = "3682--3683",

journal = "Infection and Immunity",

issn = "0019-9567",

publisher = "American Society for Microbiology",

number = "6",

}

TY - JOUR

T1 - Isolation of a human monoclonal antibody with strong neutralizing activity against diphtheria toxin

AU - Kakita, Mai

AU - Takahashi, Tsuyoshi

AU - Komiya, Takako

AU - Iba, Yoshitaka

AU - Tsuji, Takao

AU - Kurosawa, Yoshikazu

AU - Takahashi, Motohide

N1 - Funding Information: The assistance of residents of the Tahuayo, Amazon, Ucayali, Putumayo, and Nanay Rivers is gratefully acknowledged. Special thanks go to colleagues and staff at IIAP-Iquitos, IIAP-Pucallpa, INIA-Iquitos, PNCC-Iquitos, PNCCPucallpa, INADE-Iquitos, INRENA-El Estrecho, and INPA-Manaus. Mario Pinedo, Jorge Soplin, Dennis Huayllahua and Pilar Diaz are acknowledged for their exceptional support and collaboration. Field research was generously funded by the Rainforest Conservation Fund (RCF) of Chicago, Illinois; the Dallas Zoological Society, and the Tropical Conservation and Development Program of the University of Florida.

PY - 2006/6

Y1 - 2006/6

N2 - We isolated a human monoclonal antibody against diphtheria toxin (DT). It bound to fragment B with a binding activity (Kd) of 3.01 nM. The neutralizing activity assayed by the rabbit skin test was estimated to be 73,600 IU/g. This could be used as a therapeutic drug against DT in place of the traditional equine sera.

AB - We isolated a human monoclonal antibody against diphtheria toxin (DT). It bound to fragment B with a binding activity (Kd) of 3.01 nM. The neutralizing activity assayed by the rabbit skin test was estimated to be 73,600 IU/g. This could be used as a therapeutic drug against DT in place of the traditional equine sera.

UR - https://www.scopus.com/pages/publications/33646948222

UR - https://www.scopus.com/pages/publications/33646948222#tab=citedBy

U2 - 10.1128/IAI.01731-05

DO - 10.1128/IAI.01731-05

M3 - Article

C2 - 16714603

AN - SCOPUS:33646948222

SN - 0019-9567

VL - 74

SP - 3682

EP - 3683

JO - Infection and Immunity

JF - Infection and Immunity

IS - 6

ER -

Isolation of a human monoclonal antibody with strong neutralizing activity against diphtheria toxin

抄録

UN SDG

All Science Journal Classification (ASJC) codes

文献へのアクセス

他のファイルとリンク

フィンガープリント

引用スタイル