Isolation of antigen/antibody complexes through organic solvent (ICOS) method

Yasushi Akahori, Gene Kurosawa, Mariko Sumitomo, Miwa Morita, Chiho Muramatsu, Keiko Eguchi, Miho Tanaka, Kazuhiro Suzuki, Mototaka Sugiura, Yoshitaka Iba, Atsushi Sugioka, Yoshikazu Kurosawa

研究成果: Article査読

18 被引用数 (Scopus)

抄録

We developed a method termed ICOS (isolation of antigen-antibody complexes through organic solvent) for comprehensive isolation of monoclonal antibodies (mAbs) bound to molecules on the cell surface. By mixing a large number of phage particles of an antibody (Ab) library with living cells, antigen (Ag)-Ab complexes were formed on the cell surface. The mixture was overlaid on organic solution in a tube and subjected to centrifugation. Phages bound to cells were recovered from the precipitate. The phage fraction isolated turned out to contain mAbs that bind to very heterogeneous epitopes and show strong binding activity to Ags. The ICOS method was applied to isolation of human mAbs that may be therapeutic against cancers. Sixty percent of clones isolated by the screening of a phage Ab library against cancer cells turned out to bind to various kinds of tumor-associated Ags. The precise protocol of ICOS method and the rationale of efficient screening were described.

本文言語English
ページ(範囲)832-835
ページ数4
ジャーナルBiochemical and Biophysical Research Communications
378
4
DOI
出版ステータスPublished - 23-01-2009

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Isolation of antigen/antibody complexes through organic solvent (ICOS) method」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル