Isolation of multipotent stem cells from mouse adipose tissue

Naoki Yamamoto, Hirohiko Akamatsu, Seiji Hasegawa, Takaaki Yamada, Satoru Nakata, Mahito Ohkuma, Ei Ichi Miyachi, Tohru Marunouchi, Kayoko Matsunaga

研究成果: Article査読

134 被引用数 (Scopus)

抄録

Background: Embryonic stem (ES) cells, bone marrow, adipose tissue or other genetically modified stem cells are being widely used in basic research in the field of regenerative medicine. However, there is no specific surface antigen that can be used as a marker of multipotent stem cells. Objective: We tried to isolate and collect putative multipotent stem cells from mouse subcutaneous adipose tissue using the p75 neurotrophin receptor (p75NTR) as a marker. Methods: Adipose tissue was processed for immunostaining using antibodies anti-CD90, anti-CD105 and anti-Sca-1 as general mesenchymal stem cell (MSC) markers, and anti-p75NTR, an epithelial stem cell and MSC marker. Subsequently, the expression of cell surface markers in adipose tissue-derived stromal vascular fraction culture cells (ADSVF cells) was examined by flow cytometry (fluorescence-activated cell sorting: FACS). Finally, ADSVF cells positive for p75NTR were sorted and cultured to induce their differentiation into adipocytes, osteoblasts, chondrocytes, smooth muscle cells and neuronal cells. Results: Cells positive for several of these markers were found in the deep layers of adipose tissue. Among them, those positive for p75NTR differentiated into adipocytes, osteoblasts, chondrocytes, smooth muscle cells and neuronal cells. The rate of differentiation into adipocytes, osteoblasts and neuronal cells was higher for p75NTR-positive cells than for p75NTR-negative cells. Conclusions: p75NTR proved to be a useful marker to isolate adipose tissue-derived stem cells (ASCs).

本文言語English
ページ(範囲)43-52
ページ数10
ジャーナルJournal of Dermatological Science
48
1
DOI
出版ステータスPublished - 10-2007

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 皮膚病学

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