Isolation of the interacting molecules with GEX-3 by a novel functional screening

Daisuke Tsuboi, Hiroshi Qadota, Katsuhisa Kasuya, Mutsuki Amano, Kozo Kaibuchi

研究成果: Article査読

27 被引用数 (Scopus)

抄録

To screen for important molecules that interact with a gene of interest in Caenorhabditis elegans (C. elegans), we established a novel functional screening system using the yeast two-hybrid system with the RNA interference technique. Our screening system makes it possible to identify the molecular machinery involved in the function of a gene of interest starting with the cDNA of this gene. As a model case, we examined the molecular machinery involved in the function of GEX-3, an essential factor of tissue morphogenesis. We identified many interacting molecules by yeast two-hybrid screening and could detect some functional interactions using this novel functional screening system.

本文言語English
ページ(範囲)697-701
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
292
3
DOI
出版ステータスPublished - 2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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