Cone horizontal cells from the catfish retina extend out processes after a few days in culture that sometimes contact adjacent cone horizontal cells. Two types of specialized junctions were observed by electron microscopy along the newly formed contact areas. One junctional type consisted of prominent electron-dense material along and just under the plasma membrane of one or both of the contacting elements. Sometimes vesicle clusters were associated with these junctions. The other type of junction showed some electron-dense material along the membranes of both processes and patchy areas of close membrane apposition resembling gap junctions. In about half of the cases tested, electrical coupling was detected between cone horizontal cells that had made contact in culture. In no case was the coupling as tight as is typically found between horizontal cells that had formed gap junctions in vivo.
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