Katanin p60 contributes to microtubule instability around the midbody and facilitates cytokinesis in rat cells

Moe Matsuo, Tetsuhiro Shimodaira, Takashi Kasama, Yukie Hata, Ayumi Echigo, Masaki Okabe, Kazuya Arai, Yasutaka Makino, Shin Ichiro Niwa, Hideyuki Saya, Toshihiko Kishimoto

研究成果: Article査読

19 被引用数 (Scopus)

抄録

The completion of cytokinesis is crucial for mitotic cell division. Cleavage furrow ingression is followed by the breaking and resealing of the intercellular bridge, but the detailed mechanism underlying this phenomenon remains unknown. Katanin is a microtubule-severing protein comprised of an AAA ATPase subunit and an accessory subunit designated as p60 and p80, respectively. Localization of katanin p60 was observed at the midzone to midbody from anaphase to cytokinesis in rat cells, and showed a ring-shaped distribution in the gap between the inside of the contractile ring and the central spindle bundle in telophase. Katanin p60 did not bind with p80 at the midzone or midbody, and localization was shown to be dependent on microtubules. At the central spindle and the midbody, no microtubule growth plus termini were seen with katanin p60, and microtubule density was inversely correlated with katanin p60 density in the region of katanin p60 localization that seemed to lead to microtubule destabilization at the midbody. Inhibition of katanin p60 resulted in incomplete cytokinesis by regression and thus caused the appearance of binucleate cells. These results suggest that katanin p60 contributes to microtubule instability at the midzone and midbody and facilitates cytokinesis in rat cells.

本文言語English
論文番号e80392
ジャーナルPloS one
8
11
DOI
出版ステータスPublished - 26-11-2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)
  • 一般

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