Kir6.2-deficient mice develop somatosensory dysfunction and axonal loss in the peripheral nerves

Glucose-responsive ATP-sensitive potassium channels (K_ATP) are expressed in a variety of tissues including nervous systems. The depolarization of the membrane potential induced by glucose may lead to hyperexcitability of neurons and induce excitotoxicity. However, the roles of K_ATP in the peripheral nervous system (PNS) are poorly understood. Here, we determine the roles of K_ATP in the PNS using K_ATP-deficient (Kir6.2-deficient) mice. We demonstrate that neurite outgrowth of dorsal root ganglion (DRG) neurons was reduced by channel closers sulfonylureas. However, a channel opener diazoxide elongated the neurite. K_ATP subunits were expressed in mouse DRG, and expression of certain subunits including Kir6.2 was increased in diabetic mice. In Kir6.2-deficient mice, the current perception threshold, thermal perception threshold, and sensory nerve conduction velocity were impaired. Electron microscopy revealed a reduction of unmyelinated and small myelinated fibers in the sural nerves. In conclusion, K_ATP may contribute to the development of peripheral neuropathy.