@article{df9a854c6f6a4ba090ff58fdeeb049fe,
title = "Knockdown of ZEB1, a master epithelial-to-mesenchymal transition (EMT) gene, suppresses anchorage-independent cell growth of lung cancer cells",
abstract = "We found that among four master epithelial-to-mesenchymal transition (EMT)-inducing genes (ZEB1, SIP1, Snail, and Slug) ZEB1expression was most significantly correlated with the mesenchymal phenotype (high Vimentin and low E-cadherin expression) in non-small cell lung cancer (NSCLC) cell lines and tumors. Furthermore, ZEB1 knockdown with RNA interference in three NSCLC cell lines with high ZEB1 expression suppressed to varying degrees mass culture growth and liquid colony formation but in all cases dramatically suppressed soft agar colony formation. In addition, ZEB1 knockdown induced apoptosis in one of the three lines, indicating that the growth inhibitory effects of ZEB1 knockdown occurs in part through the activation of the apoptosis pathway. These results suggest that inhibiting ZEB1 function may be an attractive target for NSCLC therapeutic development.",
author = "Yoshihiro Takeyama and Mitsuo Sato and Mihoko Horio and Tetsunari Hase and Kenya Yoshida and Toshihiko Yokoyama and Harunori Nakashima and Naozumi Hashimoto and Yoshitaka Sekido and Gazdar, {Adi F.} and Minna, {John D.} and Masashi Kondo and Yoshinori Hasegawa",
note = "Funding Information: This work was supported by Grant-in-Aid for Scientific Research (C) 20590919 (to M.S.), Grant-in-Aid for Scientific Research (C) 20590918 (to M.K), and Grant-in-Aid for Scientific Research (B) 21390257 (to Y.H.) from Japan Society for the Promotion of Science, Global COE program in Nagoya University Graduate School of Medicine funded by Japan{\textquoteright}s Ministry of Education, Culture, Sports, Science and Technology, NCI Special Program of Research Excellence in lung Cancer (SPORE P50CA70907), and DOD PROSPECT (to JDM and AFG). We thank Dr. David S. Shames for his helpful comments and discussion on this study.",
year = "2010",
month = oct,
doi = "10.1016/j.canlet.2010.04.008",
language = "English",
volume = "296",
pages = "216--224",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "2",
}