Latent infection of human herpesvirus 6 in astrocytoma cell line and alteration of cytokine synthesis

Tetsushi Yoshikawa, Yoshizo Asano, Shiho Akimoto, Takao Ozaki, Takuya Iwasaki, Takeshi Kurata, Fumi Goshima, Yukihiro Nishiyama

研究成果: Article査読

65 被引用数 (Scopus)

抄録

In order to study the pathogenesis of HHV-6 infection in central nervous system disorders, U251 cell line was infected with freshly isolated variant B HHV-6. Although IEA/ex 3 antigen (immediate early protein) was detected in infected cell nuclei, neither the presence of OHV-3 antigen (late antigen) nor production of infectious virus was demonstrated. These results indicate that abortive infection was established in the cells. After viral infection, the viral genome copy in the infected cells gradually decreased and became stable after 6 cell passages. At that point, HHV-6 gene expression was restricted to only 2 immediate early genes (U90 and U94). However, 12-O-tetra-decanoyl (TPA) treatment induced transcription of other genes (U31 and U39) by the 10th cell passage, indicating HHV-6 reactivation. Moreover, production of two proinflamatory cytokines (IL-6 and IL-1β) was up-regulated by the presence of the HHV-6 genome and TPA-induced activation of the viral transcripts.

本文言語English
ページ(範囲)497-505
ページ数9
ジャーナルJournal of Medical Virology
66
4
DOI
出版ステータスPublished - 2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • ウイルス学
  • 感染症

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