Leucine zipper protein 1 (LUZP1) regulates the constriction velocity of the contractile ring during cytokinesis

Toshinori Hyodo, Eri Asano-Inami, Satoko Ito, Mai Sugiyama, Akihiro Nawa, Md Lutfur Rahman, Muhammad Nazmul Hasan, Yuko Mihara, Vu Quang Lam, Sivasundaram Karnan, Akinobu Ota, Shinobu Tsuzuki, Michinari Hamaguchi, Yoshitaka Hosokawa, Hiroyuki Konishi

研究成果: ジャーナルへの寄稿学術論文査読

2 被引用数 (Scopus)

抄録

There has been a great deal of research on cell division and its mechanisms; however, its processes still have many unknowns. To find novel proteins that regulate cell division, we performed the screening using siRNAs and/or the expression plasmid of the target genes and identified leucine zipper protein 1 (LUZP1). Recent studies have shown that LUZP1 interacts with various proteins and stabilizes the actin cytoskeleton; however, the function of LUZP1 in mitosis is not known. In this study, we found that LUZP1 colocalized with the chromosomal passenger complex (CPC) at the centromere in metaphase and at the central spindle in anaphase and that these LUZP1 localizations were regulated by CPC activity and kinesin family member 20A (KIF20A). Mass spectrometry analysis identified that LUZP1 interacted with death-associated protein kinase 3 (DAPK3), one regulator of the cleavage furrow ingression in cytokinesis. In addition, we found that LUZP1 also interacted with myosin light chain 9 (MYL9), a substrate of DAPK3, and comprehensively inhibited MYL9 phosphorylation by DAPK3. In line with a known role for MYL9 in the actin-myosin contraction, LUZP1 suppression accelerated the constriction velocity at the division plane in our time-lapse analysis. Our study indicates that LUZP1 is a novel regulator for cytokinesis that regulates the constriction velocity of the contractile ring.

本文言語英語
ページ(範囲)927-944
ページ数18
ジャーナルFEBS Journal
291
5
DOI
出版ステータス出版済み - 03-2024
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

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