Lipocalin-type prostaglandin D synthase and egg white cystatin react with IgE antibodies from children with egg allergy

Makiko Suzuki, Hidehiko Fujii, Hidetsugu Fujigaki, Shinji Shinoda, Kanako Takahashi, Kuniaki Saito, Hisayasu Wada, Masumi Kimoto, Naomi Kondo, Mitsuru Seishima

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Background: Ovalbumin, ovomucoid, ovotransferrin, lysozyme, and ovomucin are known to be major allergens found in egg white. Egg white protein is composed of over 30 proteins; many of which have neither been identified nor their allergenicities characterized. This study set out to analyze whether unknown proteins that bind to IgE antibodies in serum from patients with egg allergy exist in egg white. Methods: Diluted egg white proteins were separated by 2-dimensional (2-D) gel electrophoresis. Immunola-beling was performed on individual patient sera from 19 child patients with egg white allergy and 11 negative control subjects. Spots of egg white proteins that bound to the patients' IgE were identified by mass spectrometry-based proteomics. Results: Egg white proteins were separated into 63 spots. Twenty-five of the 63 reacted with egg allergy patients' sera, and 10 of the 25 reactive spots showed IgE-reactivity to controls as well. Specific bindings to the IgE from egg allergy patients were found in 15 spots; one of which was confirmed as ovotransferrin. Among the other 14 protein spots, egg white cystatin and lipocalin-type prostaglandin D synthase (L-PGDS) were newly identified proteins that reacted with IgE in patients with egg allergy. Conclusions: We demonstrated that L-PGDS and cystatin reacted with serum IgE in patients with egg allergy. Our proteomics-based analysis in egg white gives a comprehensive map of proteins bound with IgE and should assist in enabling more accurate diagnoses and recommendations of desensitizing treatments for individual patients.

本文言語English
ページ(範囲)175-183
ページ数9
ジャーナルAllergology International
59
2
DOI
出版ステータスPublished - 2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy

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