Live imaging of fetal intra-abdominal organs using two-photon laser-scanning microscopy

Yuhki Koike, Bo Li, Yong Chen, Hiromu Miyake, Carol Lee, Lijun Chi, Richard Wu, Mikihiro Inoue, Keiichi Uchida, Masato Kusunoki, Paul Delgado-Olguin, Agostino Pierro

研究成果: Chapter

3 被引用数 (Scopus)

抄録

The processes by which the intra-abdominal organ circulatory system develops in the embryo and during organogenesis are unclear. Previous studies have used fixed tissues to study the development of abdominal organ vasculature in the embryo; however, the intravital circulation of intra-abdominal organs in rodent fetal development has not been studied. This protocol describes a system that uses two-photon laser-scanning microscopy (TPLSM) for real-time observation and quantification of normal and pathologic live fetal intra-abdominal dynamics while the fetus is still connected to the mother via the umbilical cord.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ63-69
ページ数7
DOI
出版ステータスPublished - 2018
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
1752
ISSN(印刷版)1064-3745

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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