Localization of Rabphilin-3A, a Putative Target Protein for Rab3A, at the Sites of Ca2+-Dependent Exocytosis in PC12 Cells

K. Wada, A. Mizoguchi, K. Kaibuchi, H. Shirataki, C. Ide, Y. Takai

研究成果: Article査読

17 被引用数 (Scopus)

抄録

Rab3A/Smg 25A, a small GTP-binding protein, is highly concentrated in presynapse of neurons and implicated in neurotransmitter release. We have recently identified a putative target protein for Rab3A, isolated its cDNA, and designated it as Rabphilin-3A. To examine whether Rabphilin-3A as well as Rab3A is localized at the sites of Ca2+-dependent exocytosis, we investigated here localization of Rabphilin-3A and Rab3A in comparison with the sites of exocytosis ira the differentiated PC12 cells. Rabphilin-3A as well as Rab3A was highly concentrated at the tips of the neurites where Ca2+-dependent exocytosis took place. Inversely, neither Rabphilin-3A nor Rab3A was concentrated at the tips of the neurites where Ca2+-dependent exocytosis did not take place. These results suggest that Rabphilin-3A as well as Rab3A constitutes a part of the machinery necessary for neurotransmitter release.

本文言語English
ページ(範囲)158-165
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
198
1
DOI
出版ステータスPublished - 15-01-1994
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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