Longitudinal study of circulating miR-122 in a rat model of non-alcoholic fatty liver disease

Hiroya Yamada, Koji Ohashi, Koji Suzuki, Eiji Munetsuna, Yoshitaka Ando, Mirai Yamazaki, Hiroaki Ishikawa, Naohiro Ichino, Ryouji Teradaira, Shuji Hashimoto

研究成果: ジャーナルへの寄稿学術論文査読

65 被引用数 (Scopus)


Background: Circulating microRNAs (miRs) may be promising biomarkers for several diseases. We previously found that miR-122 can function as a biomarker for non-alcoholic fatty liver disease (NAFLD). However, little is known regarding the time course of circulating miR-122 levels during the development of NAFLD. Here, we examined circulating miR-122 levels using a rat model of NAFLD. Methods: To clarify changes in serum levels of miR-122 during development of NAFLD, experimental rats were fed a high-fat diet (HFD) for 2-10. weeks, while control rats received standard chow. Serum and liver tissue was collected from all animals at 2, 6, and 10. weeks of feeding. Clinical laboratory parameters (cholesterol, TG, AST, ALT, NEFA) were determined by biochemistry analyzer. Hepatic lipid accumulation was estimated by Oil red O staining. Circulating miR-122 levels were then measured by real-time polymerase chain reaction. Results: Over the 10. weeks of feeding, body weight, total liver lipids, and liver and serum triacylglycerol were increased in the HFD group compared to the control group. However, no significant changes in serum alanine aminotransferase activity were observed, suggesting that NAFLD status was mild. In contrast, we observed drastic up-regulation of circulating miR-122 levels. Our findings suggest that serum miR-122 level is indeed useful for assessing early NAFLD and might be superior to clinical markers traditionally used to monitor hepatic disease.

ジャーナルClinica Chimica Acta
出版ステータス出版済み - 2015

All Science Journal Classification (ASJC) codes

  • 生化学
  • 臨床生化学
  • 生化学、医学


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