Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B

Masaru Ihira, Akane Ohta, Ken Sugata, Sadao Suga, Yoshizo Asano, Tetsushi Yoshikawa

研究成果: Article査読

16 被引用数 (Scopus)

抄録

Genotyping of human herpesvirus 6 (HHV-6) is important clinically, particularly for the diagnosis of neurological diseases. The objective of this study was to establish a rapid HHV-6 genotyping method using the loop-mediated isothermal amplification (LAMP) method. An AccI site is located in the target sequence of HHV-6 B, but not in HHV-6 A. LAMP products were digested with the AccI enzyme and then separated by agarose gel electrophoresis to differentiate the digest pattern of the two variants. The fragment patterns were clearly different between HHV-6 A and B. In order to evaluate the reliability of this HHV-6 genotyping method for use in the clinical laboratory, serum samples from 20 patients with either primary HHV-6 infection or viral reactivation were collected and analyzed. HHV-6 DNA was amplified directly from the serum samples and all 20 LAMP products were positive for HHV-6 B.

本文言語English
ページ(範囲)223-225
ページ数3
ジャーナルJournal of Virological Methods
154
1-2
DOI
出版ステータスPublished - 12-2008

All Science Journal Classification (ASJC) codes

  • Virology

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