Luciferase expression 2 years after DNA injection in glass catfish (Kryptopterus bicirrhus)

J. M. Dijkstra; H. Okamoto; M. Ototake; T. Nakanishi

doi:10.1006/fsim.2000.0303

Luciferase expression 2 years after DNA injection in glass catfish (Kryptopterus bicirrhus)

J. M. Dijkstra
, H. Okamoto
, M. Ototake
, T. Nakanishi

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

34 被引用数 (Scopus)

抄録

Glass catfish (Kryptopterus bicirrhus) have transparent muscles and skin. Intramuscular injection of DNA encoding luciferase into these fish induced luciferase expression that was measurable in vivo with a low light video image analyser. Expression could be detected up to at least 2 years after DNA injection. Although luciferase is not representative of all types of antigen, this study stresses the need for future studies directed to limit the period of antigen expression after DNA vaccination.

本文言語	英語
ページ（範囲）	199-202
ページ数	4
ジャーナル	Fish and Shellfish Immunology
巻	11
号	2
DOI	https://doi.org/10.1006/fsim.2000.0303
出版ステータス	出版済み - 02-2001
外部発表	はい

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All Science Journal Classification (ASJC) codes

免疫学および微生物学（その他）
水圏科学
免疫学
環境化学

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10.1006/fsim.2000.0303

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title = "Luciferase expression 2 years after DNA injection in glass catfish (Kryptopterus bicirrhus)",

abstract = "Glass catfish (Kryptopterus bicirrhus) have transparent muscles and skin. Intramuscular injection of DNA encoding luciferase into these fish induced luciferase expression that was measurable in vivo with a low light video image analyser. Expression could be detected up to at least 2 years after DNA injection. Although luciferase is not representative of all types of antigen, this study stresses the need for future studies directed to limit the period of antigen expression after DNA vaccination.",

author = "Dijkstra, \{J. M.\} and H. Okamoto and M. Ototake and T. Nakanishi",

note = "Funding Information: This study was supported by a postdoctoral fellowship for J. M. Dijkstra from the Science and Technology Agency of Japan. We would like to thank Dr K. Araki for supplying us with the pCEP4-luc plasmid and for his helpful comments concerning this manuscript.",

year = "2001",

month = feb,

doi = "10.1006/fsim.2000.0303",

language = "English",

volume = "11",

pages = "199--202",

journal = "Fish and Shellfish Immunology",

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AU - Dijkstra, J. M.

AU - Okamoto, H.

AU - Ototake, M.

AU - Nakanishi, T.

N1 - Funding Information: This study was supported by a postdoctoral fellowship for J. M. Dijkstra from the Science and Technology Agency of Japan. We would like to thank Dr K. Araki for supplying us with the pCEP4-luc plasmid and for his helpful comments concerning this manuscript.

PY - 2001/2

Y1 - 2001/2

N2 - Glass catfish (Kryptopterus bicirrhus) have transparent muscles and skin. Intramuscular injection of DNA encoding luciferase into these fish induced luciferase expression that was measurable in vivo with a low light video image analyser. Expression could be detected up to at least 2 years after DNA injection. Although luciferase is not representative of all types of antigen, this study stresses the need for future studies directed to limit the period of antigen expression after DNA vaccination.

AB - Glass catfish (Kryptopterus bicirrhus) have transparent muscles and skin. Intramuscular injection of DNA encoding luciferase into these fish induced luciferase expression that was measurable in vivo with a low light video image analyser. Expression could be detected up to at least 2 years after DNA injection. Although luciferase is not representative of all types of antigen, this study stresses the need for future studies directed to limit the period of antigen expression after DNA vaccination.

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UR - https://www.scopus.com/pages/publications/0035259203#tab=citedBy

U2 - 10.1006/fsim.2000.0303

DO - 10.1006/fsim.2000.0303

M3 - Article

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AN - SCOPUS:0035259203

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SP - 199

EP - 202

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

IS - 2

ER -

Luciferase expression 2 years after DNA injection in glass catfish (Kryptopterus bicirrhus)

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UN SDG

All Science Journal Classification (ASJC) codes

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