抄録
Remodeling of the matrix surrounding tumor cells plays a crucial role in the development and maintenance of cancer. Lysyl oxidase (LOX), a matrix remodeling factor, is induced by HIF-1α under hypoxic conditions and associated with tumor growth and metastasis. Here, we report that high cell density induces HIF-1α expression under normoxic condition, resulting in the promotion of LOX expression. This phenomenon was observed in the retinoblastoma tumor suppressor (RB)-proficient breast cancer cells but not in RB-deficient cells. In RB-proficient cancer cells, the cell cycle regulator E2F1 was down-regulated and cell cycle progression was inhibited at high density culture condition. Knockdown of E2F1 stabilized HIF-1α and promoted LOX expression, while knockdown of both E2F1 and HIF-1α prevented the up-regulation of LOX. These findings suggest that elevated cell density enhances cell cycle arrest and matrix remodeling via RB-E2F1-HIF-1α axis.
本文言語 | 英語 |
---|---|
ページ(範囲) | 9-14 |
ページ数 | 6 |
ジャーナル | Cell structure and function |
巻 | 38 |
号 | 1 |
DOI | |
出版ステータス | 出版済み - 2013 |
外部発表 | はい |
All Science Journal Classification (ASJC) codes
- 生理学
- 分子生物学
- 細胞生物学