Macrophage-activating factor extracted from mycoplasmas

Morio Takema, Shogo Oka, Kazuko Uno, Shinji Nakamura, Hitoshi Arita, Katsuya Tawara, Kayo Inaba, Shigeru Muramatsu

研究成果: ジャーナルへの寄稿学術論文査読

7 被引用数 (Scopus)

抄録

Mycoplasmas (M. gallisepticum, chicken mycoplasmas), in concert with interferon γ (IFNγ), were effective in activating macrophages (Mθ) to be tumoricidal. The Mθ-activating capacity of mycoplasmas was maintained after treatment with heat, 0.1 M NaOH, 1 M HC1, or trypsin. Mθ-activating factor was extracted from mycoplasmas with chloroform/methanol and water (Mf-B). Mf-B was also effective in activating Mθ in the presence of IFNγ. The threshold dose of Mf-B for Mθ of ordinary C3H/He mice and that for those of C3H/HeJ mice, the latter being known to be low responders to bacterial lipopolysaccharide, were actually the same. This seems to indicate that the effectiveness of Mf-B was not attributable to possibly contaminating lipopolysaccharides, and that the pathway of activity of Mf-B is different from that of lipopolysaccharides. Since the Mθ-activating principle was only a very small part of Mf-B, we have not yet succeeded in identifying it, but there was no evidence that it was protein, nucleic acid, sugar, or lipid. The cytotoxicity of Mθ activated by Mf-B plus IFNγ was dependent on l-arginine in the culture, suggesting that arginine metabolites are involved in Mθ cytotoxicity. Mf-B induced a small amount of tumor necrosis factor in Mθ, and this induction was markedly enhanced by IFNγ.

本文言語英語
ページ(範囲)39-44
ページ数6
ジャーナルCancer Immunology Immunotherapy
33
1
DOI
出版ステータス出版済み - 01-1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学
  • 腫瘍学
  • 癌研究

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