Measurement of the transport activities of bile salt export pump using LC-MS

Kana Yamaguchi, Tsuyoshi Murai, Hikaru Yabuuchi, Takao Kurosawa

研究成果: Article査読

17 被引用数 (Scopus)

抄録

The high performance liquid chromatography-electrospray ionization-mass spectrometry method has been applied to the measurement of bile acid transport activities in membrane vesicles obtained from a human bile salt export pump expressing Sf9 cells. The amounts of bile acids transported using the human bile salt export pump expressing Sf9 cells were determined using liquid chromatography-electrospray ionization-mass spectrometry method and the values of the kinetic parameters were determined to be comparable with those obtained using radioisotope-labeled substrates. The developed method was highly useful for the measurements of bile acid transport activities. 2009

本文言語English
ページ(範囲)1155-1158
ページ数4
ジャーナルAnalytical Sciences
25
9
DOI
出版ステータスPublished - 09-2009

All Science Journal Classification (ASJC) codes

  • 分析化学

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