TY - JOUR
T1 - Measurement of Varicella-Zoster Virus (VZV)-specific cell-mediated immunity
T2 - Comparison between VZV skin test and interferon-γ enzyme-linked immunospot assay
AU - Sadaoka, Kay
AU - Okamoto, Shigefumi
AU - Gomi, Yasuyuki
AU - Tanimoto, Takeshi
AU - Ishikawa, Toyokazu
AU - Yoshikawa, Tetsushi
AU - Asano, Yoshizo
AU - Yamanishi, Koichi
AU - Mori, Yasuko
N1 - Funding Information:
Received 24 March 2008; accepted 12 May 2008; electronically published 5 September 2008. Potential conflicts of interest: Y.G., T.T., and T.I. are employees of Biken, which manufactures skin test antigen. All other authors report no potential conflicts. Presented in part: 33rd International Herpesvirus Workshop, Estoril, Portugal, 27 July–1 August 2008 (poster 5.42). Financial support: Ministry of Health, Labor, and Welfare of Japan (Research Promotion of Emerging and Reemerging Infectious Diseases Grant H18-Shinko-013 to Y.M. and T.Y.). a K.S. and S.O. contributed equally to this work. Reprints or correspondence: Dr. Yasuko Mori, Laboratory of Virology and Vaccinology, Div. of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, Japan ([email protected]).
PY - 2008/11/1
Y1 - 2008/11/1
N2 - Cell-mediated immunity (CMI) is critical for the prevention and control of varicella-zoster virus (VZV)-related disease. To assess CMI to VZV, a varicella skin test and interferon-γ enzyme-linked immunospot (ELISPOT) assay were both performed in healthy volunteers, and the results were compared. A total of 151 subjects were examined: 16 aged 20-29 years, 26 aged 30-39 years, 18 aged 40-49 years, 73 aged 50-59 years, and 18 aged 60-69 years. All were seropositive by a glycoprotein antigen- based enzyme-linked immunosorbent assay (gpELISA). Skin test reactivity was significantly correlated with the ELISPOT count, and both decreased with increasing age, indicating an age-dependent decline in CMI to VZV. In contrast, the antibody titer obtained by the gpELISA did not correlate with skin test reactivity. The results suggest that the skin test and ELISPOT assay are both reliable for assessing CMI to VZV and can easily be applied to screen individuals susceptible to the development of herpes zoster.
AB - Cell-mediated immunity (CMI) is critical for the prevention and control of varicella-zoster virus (VZV)-related disease. To assess CMI to VZV, a varicella skin test and interferon-γ enzyme-linked immunospot (ELISPOT) assay were both performed in healthy volunteers, and the results were compared. A total of 151 subjects were examined: 16 aged 20-29 years, 26 aged 30-39 years, 18 aged 40-49 years, 73 aged 50-59 years, and 18 aged 60-69 years. All were seropositive by a glycoprotein antigen- based enzyme-linked immunosorbent assay (gpELISA). Skin test reactivity was significantly correlated with the ELISPOT count, and both decreased with increasing age, indicating an age-dependent decline in CMI to VZV. In contrast, the antibody titer obtained by the gpELISA did not correlate with skin test reactivity. The results suggest that the skin test and ELISPOT assay are both reliable for assessing CMI to VZV and can easily be applied to screen individuals susceptible to the development of herpes zoster.
UR - https://www.scopus.com/pages/publications/54249132503
UR - https://www.scopus.com/pages/publications/54249132503#tab=citedBy
U2 - 10.1086/592219
DO - 10.1086/592219
M3 - Article
C2 - 18774884
AN - SCOPUS:54249132503
SN - 0022-1899
VL - 198
SP - 1327
EP - 1333
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 9
ER -
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