Mechanism of carcinogenesis induced by a veterinary antimicrobial drug, nitrofurazone, via oxidative DNA damage and cell proliferation

Yusuke Hiraku, Aki Sekine, Hiromi Nabeshi, Kaoru Midorikawa, Mariko Murata, Yoshito Kumagai, Shosuke Kawanishi

研究成果: Article査読

54 被引用数 (Scopus)

抄録

Nitrofurazone, a veterinary antimicrobial drug, causes mammary and ovarian tumors in animals. We investigated the mechanisms of carcinogenesis by nitrofurazone. Nitrofurazone significantly stimulated the proliferation of estrogen-dependent MCF-7 cells. Nitrofurazone caused Cu(II)-mediated damage to 32P-5′-end-labeled DNA fragments obtained from human genes only when cytochrome P450 reductase was added. DNA damage was inhibited by catalase and bathocuproine. DNA damage was preferably induced at the 5′-ACG- 3′ sequence, a hotspot of the p53 gene. These findings suggest that nitrofurazone metabolites are involved in tumor initiation through oxidative DNA damage and nitrofurazone itself enhances cell proliferation, leading to promotion and/or progression in carcinogenesis.

本文言語English
ページ(範囲)141-150
ページ数10
ジャーナルCancer Letters
215
2
DOI
出版ステータスPublished - 25-11-2004
外部発表はい

All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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