Mechanism of cell interactions with water-dispersed carbon nanohorns

Tatsuya Murakami, Masashi Nakatani, Masahiro Kokubo, Hirotaka Nakatsuji, Mami Inada, Hiroshi Imahori, Masako Yudasaka, Sumio Iijima, Kunihiro Tsuchida

研究成果: Article査読

4 被引用数 (Scopus)

抄録

The interaction of water-dispersed nanocarbon materials with mammalian cells has been a matter of great interest. Here, we show a possible mechanism of cell interaction with single-walled carbon nanohorns (CNHs). Individually isolated CNHs in H2O, which were noncovalently modified with a polyethylene glycol (PEG)-doxorubicin conjugate, were visualized by atomic force microscopy. Upon treatment of four types of mammalian cells with the PEG-modified CNHs (PEG-d-CNHs), only two cell lines showed a strong affinity to CNHs. The cell interaction with PEG-d-CNHs was temperature-sensitive, suggesting the involvement of endocytosis in it. The CNHs were clearly observed on the ruffled plasma membranes as well as in the membrane compartments inside the cells by electron microscopy. Cell sorter analysis demonstrated that this cell interaction with PEG-d-CNHs was effectively inhibited by treatment with cytochalasin B, an inhibitor of membrane ruffling and macropinocytosis. Under our experimental conditions, no significant cytotoxicity was detected. These data suggest that macropinocytosis after membrane ruffling is responsible for the cell interaction.

本文言語English
ページ(範囲)402-407
ページ数6
ジャーナルNanoscience and Nanotechnology Letters
5
3
DOI
出版ステータスPublished - 03-2013

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

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