Mechanism of phospholipase D activation induced by prostaglandin D2 in osteoblast-like cells: Function of Ca2+/calmodulin

Yoko Imamura, Osamu Kozawa, Atsushi Suzuki, Yasuko Watanabe, Hidehiko Saito, Yutaka Oiso

研究成果: Article査読

15 被引用数 (Scopus)


Prostaglandin D2 (PGD2) stimulated the formation of choline in a dose-dependent manner in the range between 10 nM and 10 μM. The effect of PGD2 on the formation of inositol phosphates (EC50 was 20 nM) was more potent than that on the formation of choline (EC50 was 0.5 μM). The formation of choline stimulated by a combination of PGD2 and 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, was additive. Staurosporine, an inhibitor for protein kinases, enhanced the PGD2-induced formation of choline, but H-7, another inhibitor for protein kinases, had little effect. PGD2 stimulated Ca2+ influx from extracellular space dose-dependently. The depletion of extracellular Ca2+ by EGTA reduced the PGD2-induced formation of choline. W-7 and trifluoperazine dihydrochloride, antagonists of calmodulin, dose-dependently inhibited the PGD2-induced choline formation. These results strongly suggest that PGD2 activates phospholipase D in a Ca2+/calmodulin dependent manner in osteoblast-like cells, and that protein kinase C is not essential for the PGD2-induced activation of phospholipase D.

ジャーナルCellular Signalling
出版ステータスPublished - 01-1995

All Science Journal Classification (ASJC) codes

  • 細胞生物学


「Mechanism of phospholipase D activation induced by prostaglandin D<sub>2</sub> in osteoblast-like cells: Function of Ca<sup>2+</sup>/calmodulin」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。