Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes

Ryosuke Kikuchi, Sayaka Sobue, Masashi Murakami, Hiromi Ito, Ami Kimura, Takashi Iwasaki, Shuji Shibayama, Akira Takagi, Tetsuhito Kojima, Motoshi Suzuki, Yoshiko Banno, Yoshinori Nozawa, Takashi Murate

研究成果: Article査読

10 被引用数 (Scopus)

抄録

1α,25-Dihydroxyvitamin D3 (VitD3) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD3 increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (-260 bp to -246 bp from exon 1). Similar results were obtained by transfecting VitD3 receptor (VDR) into HEK293 cells, which are originally VitD3-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD3, VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.

本文言語English
ページ(範囲)1800-1804
ページ数5
ジャーナルFEBS Letters
581
9
DOI
出版ステータスPublished - 01-05-2007
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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